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Assay Detail

CHEMBL3370191

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-naloxone from rat mu opioid receptor expressed in HEK cells after 60 mins
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL270)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery, structure-activity relationship studies, and anti-nociceptive effects of 1-phenyl-3,6,6-trimethyl-1,5,6,7-tetrahydro-4H-indazol-4-one as novel opioid receptor agonists.
Cheng MF, Ou LC, Chen SC, Chang WT, Law PY, Loh HH, Chao YS, Shih C, Yeh SH, Ueng SH.
Bioorg Med Chem (2014) 22 : 4694 -4703
PubMed 25087049 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.45 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL80 NALOXONE 4.0 1 8.70
CHEMBL3325714 1 6.33
CHEMBL3325715 1 6.21
CHEMBL3325716 1 6.13
CHEMBL3325719 1 5.89
CHEMBL3325707 1 5.41
CHEMBL3325841 1 -
CHEMBL3325710 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL80 NALOXONE Ki = 2.0 nM 8.70
CHEMBL3325714 Ki = 470.0 nM 6.33
CHEMBL3325715 Ki = 610.0 nM 6.21
CHEMBL3325716 Ki = 740.0 nM 6.13
CHEMBL3325719 Ki = 1300.0 nM 5.89
CHEMBL3325707 Ki = 3930.0 nM 5.41
CHEMBL3325841 Ki > 100000.0 nM -
CHEMBL3325710 Ki > 100000.0 nM -