Assay Detail

CHEMBL3380992

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional

Agonist activity at rat S1P1 receptor assessed as cAMP accumulation by HTRF assay

ChEMBL ↗

Free Research Context This assay will appear in recent viewed items on this browser. Open the demo workspace to preview project-only review queues.

Research home Demo workspace

Target

Sphingosine 1-phosphate receptor 1 (CHEMBL1914263) ↗

Open DB target context

Use one target anchor to keep assay intent, target linkage, and tested compound review aligned.

Loading context

Sphingosine 1-phosphate receptor 1 (CHEMBL1914263) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target Sphingosine 1-phosphate receptor 1

CHEMBL1914263

Anchored from Assay target.

Protein identity Sphingosine 1-phosphate receptor 1

Disease frame Disease evidence pending

Open the live target snapshot or evidence card before reviewing assay metadata so target fit and disease framing stay reusable.

Review focus Open DB context pending

Open the live target snapshot before relying on this target frame across surface handoff.

Open target snapshot Open evidence card Search with this context

Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Functional
Organism	Rattus norvegicus
Confidence	9 — Direct single protein target
Curated By	Autocuration

Target

Sphingosine 1-phosphate receptor 1 (CHEMBL1914263) ↗

Type	SINGLE PROTEIN
Organism	Rattus norvegicus

Publication

Discovery of APD334: Design of a Clinical Stage Functional Antagonist of the Sphingosine-1-phosphate-1 Receptor.

Buzard DJ, Kim SH, Lopez L, Kawasaki A, Zhu X, Moody J, Thoresen L, Calderon I, Ullman B, Han S, Lehmann J, Gharbaoui T, Sengupta D, Calvano L, Montalban AG, Ma YA, Sage C, Gao Y, Semple G, Edwards J, Barden J, Morgan M, Chen W, Usmani K, Chen C, Sadeque A, Christopher RJ, Thatte J, Fu L, Solomon M, Mills D, Whelan K, Al-Shamma H, Gatlin J, Le M, Gaidarov I, Anthony T, Unett DJ, Blackburn A, Rueter J, Stirn S, Behan DP, Jones RM.
ACS Med Chem Lett (2014) 5 : 1313 -1317
PubMed 25516790 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
EC50	1	9.49	9.49

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL3358920	ETRASIMOD	4.0	1	9.49

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL3358920	ETRASIMOD	EC50	=	0.32	nM	9.49