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Assay Detail

CHEMBL3404590

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine kidney non-specific alkaline phosphatase using CDP-star chemiluminescent substrate assessed as change in luminescence by spectrophotometric method
38
Total Activities
19
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alkaline phosphatase, tissue-nonspecific isozyme (CHEMBL2406894)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Diarylsulfonamides and their bioisosteres as dual inhibitors of alkaline phosphatase and carbonic anhydrase: Structure activity relationship and molecular modelling studies.
Al-Rashida M, Ejaz SA, Ali S, Shaukat A, Hamayoun M, Ahmed M, Iqbal J.
Bioorg Med Chem (2015) 23 : 2435 -2444
PubMed 25865133 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.43 6.94
Ki 19 5.79 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3403324 2 7.30
CHEMBL3403327 2 7.22
CHEMBL182659 2 7.16
CHEMBL3403329 2 6.89
CHEMBL3403320 2 6.52
CHEMBL3403317 2 6.04
CHEMBL3403325 2 6.02
CHEMBL511186 2 5.90
CHEMBL23922 2 5.86
CHEMBL1454 LEVAMISOLE 4.0 2 5.74
CHEMBL3403318 2 5.40
CHEMBL3403321 2 5.35
CHEMBL3403322 2 4.79
CHEMBL23559 2 4.78
CHEMBL1607835 2 4.75
CHEMBL3403319 2 4.60
CHEMBL3403323 2 4.04
CHEMBL3403326 2 -
CHEMBL3403328 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3403324 Ki = 50.0 nM 7.30
CHEMBL3403327 Ki = 60.0 nM 7.22
CHEMBL182659 Ki = 70.0 nM 7.16
CHEMBL3403324 IC50 = 115.0 nM 6.94
CHEMBL3403329 Ki = 128.0 nM 6.89
CHEMBL3403327 IC50 = 150.0 nM 6.82
CHEMBL182659 IC50 = 180.0 nM 6.75
CHEMBL3403320 Ki = 299.0 nM 6.52
CHEMBL3403329 IC50 = 310.0 nM 6.51
CHEMBL3403320 IC50 = 613.0 nM 6.21
CHEMBL3403317 Ki = 918.0 nM 6.04
CHEMBL3403325 Ki = 950.0 nM 6.02
CHEMBL511186 Ki = 1250.0 nM 5.90
CHEMBL23922 Ki = 1390.0 nM 5.86
CHEMBL1454 LEVAMISOLE Ki = 1810.0 nM 5.74
CHEMBL3403325 IC50 = 2240.0 nM 5.65
CHEMBL3403317 IC50 = 2870.0 nM 5.54
CHEMBL511186 IC50 = 2940.0 nM 5.53
CHEMBL23922 IC50 = 3260.0 nM 5.49
CHEMBL3403318 Ki = 3960.0 nM 5.40
CHEMBL3403321 Ki = 4510.0 nM 5.35
CHEMBL3403318 IC50 = 10300.0 nM 4.99
CHEMBL3403322 Ki = 16200.0 nM 4.79
CHEMBL23559 Ki = 16610.0 nM 4.78
CHEMBL1607835 Ki = 17590.0 nM 4.75
CHEMBL1454 LEVAMISOLE IC50 = 19210.0 nM 4.72
CHEMBL3403321 IC50 = 22500.0 nM 4.65
CHEMBL3403319 Ki = 25100.0 nM 4.60
CHEMBL1607835 IC50 = 41200.0 nM 4.38
CHEMBL3403322 IC50 = 55600.0 nM 4.25
CHEMBL3403319 IC50 = 58600.0 nM 4.23
CHEMBL23559 IC50 = 70100.0 nM 4.15
CHEMBL3403323 Ki = 91870.0 nM 4.04
CHEMBL3403326 IC50 - -
CHEMBL3403323 IC50 = 221900.0 nM -
CHEMBL3403326 Ki - -
CHEMBL3403328 Ki - -
CHEMBL3403328 IC50 - -