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Assay Detail

CHEMBL3420196

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Functional
Partial agonist activity at human muscarinic M1 acetylcholine receptor expressed in CHO cells assessed as increase in IP1 accumulation incubated for 30 mins by FRET based HTRF assay
14
Total Activities
14
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M1 (CHEMBL216)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Rational design of partial agonists for the muscarinic m1 acetylcholine receptor.
Chen X, Klöckner J, Holze J, Zimmermann C, Seemann WK, Schrage R, Bock A, Mohr K, Tränkle C, Holzgrabe U, Decker M.
J Med Chem (2015) 58 : 560 -576
PubMed 25478907 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 6.92 8.69
pEC50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3121473 1 8.69
CHEMBL3414842 1 7.81
CHEMBL3414840 1 7.49
CHEMBL3414838 1 7.24
CHEMBL3414844 1 7.23
CHEMBL3414847 1 7.22
CHEMBL3414839 1 7.20
CHEMBL3414841 1 6.75
CHEMBL3414843 1 6.52
CHEMBL667 ACETYLCHOLINE 4.0 1 6.43
CHEMBL3414845 1 6.27
CHEMBL550 PILOCARPINE 4.0 1 6.08
CHEMBL608112 1 5.08
CHEMBL3414846 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3121473 EC50 = 2.042 nM 8.69
CHEMBL3414842 EC50 = 15.49 nM 7.81
CHEMBL3414840 EC50 = 32.36 nM 7.49
CHEMBL3414838 EC50 = 57.54 nM 7.24
CHEMBL3414844 EC50 = 58.88 nM 7.23
CHEMBL3414847 EC50 = 60.26 nM 7.22
CHEMBL3414839 EC50 = 63.1 nM 7.20
CHEMBL3414841 EC50 = 177.83 nM 6.75
CHEMBL3414843 EC50 = 302.0 nM 6.52
CHEMBL667 ACETYLCHOLINE EC50 = 371.54 nM 6.43
CHEMBL3414845 EC50 = 537.03 nM 6.27
CHEMBL550 PILOCARPINE EC50 = 831.76 nM 6.08
CHEMBL608112 EC50 = 8317.64 nM 5.08
CHEMBL3414846 pEC50 - -