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Assay Detail

CHEMBL3540944

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of recombinant CYP3A4 (unknown origin) expressed in baculosomes in presence of 1 mM NADPH
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Mechanism-based inactivation of cytochrome P450 3A4 by mibefradil through heme destruction.
Foti RS, Rock DA, Pearson JT, Wahlstrom JL, Wienkers LC.
Drug Metab Dispos (2011) 39 : 1188 -1195
PubMed 21447734 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 6.32 6.55
Kinact 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL45816 MIBEFRADIL 4.0 2 6.55
CHEMBL3526662 2 6.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL45816 MIBEFRADIL Ki = 280.0 nM 6.55
CHEMBL3526662 Ki = 820.0 nM 6.09
CHEMBL45816 MIBEFRADIL Kinact = 0.59 /min -
CHEMBL3526662 Kinact = 0.08 /min -