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Assay Detail

CHEMBL3583718

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human melatonin receptor-2 transfected in CHO cell membranes after 1 hr by GTPgammaS binding assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melatonin receptor type 1B (CHEMBL1946)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel N-Acetyl Bioisosteres of Melatonin: Melatonergic Receptor Pharmacology, Physicochemical Studies, and Phenotypic Assessment of Their Neurogenic Potential.
de la Fuente Revenga M, Fernández-Sáez N, Herrera-Arozamena C, Morales-García JA, Alonso-Gil S, Pérez-Castillo A, Caignard DH, Rivara S, Rodríguez-Franco MI.
J Med Chem (2015) 58 : 4998 -5014
PubMed 26023814 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 19 7.17 10.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL45 MELATONIN 4.0 1 10.16
CHEMBL3580908 1 8.52
CHEMBL3580910 1 7.96
CHEMBL3580919 1 7.54
CHEMBL3580915 1 7.15
CHEMBL3580907 1 7.09
CHEMBL3580926 1 6.96
CHEMBL7257 MEBUFOTENIN 2.0 1 6.95
CHEMBL3580909 1 6.88
CHEMBL3580913 1 6.53
CHEMBL3580917 1 6.34
CHEMBL3580912 1 6.30
CHEMBL3580916 1 6.05
CHEMBL3580921 1 5.96
CHEMBL3580918 1 -
CHEMBL3580911 1 -
CHEMBL3580920 1 -
CHEMBL3580922 1 -
CHEMBL3580924 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL45 MELATONIN EC50 = 0.069 nM 10.16
CHEMBL3580908 EC50 = 3.0 nM 8.52
CHEMBL3580910 EC50 = 11.0 nM 7.96
CHEMBL3580919 EC50 = 29.0 nM 7.54
CHEMBL3580915 EC50 = 71.0 nM 7.15
CHEMBL3580907 EC50 = 81.0 nM 7.09
CHEMBL3580926 EC50 = 110.0 nM 6.96
CHEMBL7257 MEBUFOTENIN EC50 = 112.0 nM 6.95
CHEMBL3580909 EC50 = 132.0 nM 6.88
CHEMBL3580913 EC50 = 295.0 nM 6.53
CHEMBL3580917 EC50 = 457.0 nM 6.34
CHEMBL3580912 EC50 = 503.0 nM 6.30
CHEMBL3580916 EC50 = 881.0 nM 6.05
CHEMBL3580921 EC50 = 1096.0 nM 5.96
CHEMBL3580918 EC50 > 1000.0 nM -
CHEMBL3580911 EC50 - -
CHEMBL3580920 EC50 > 1000.0 nM -
CHEMBL3580922 EC50 > 1000.0 nM -
CHEMBL3580924 EC50 > 1000.0 nM -