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Assay Detail

CHEMBL3590711

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of phosphorylated NPM1 recruitment to chromatic in 6 Gy irradiated human A549 cells assessed as extraction of pT199NPM1 pretreated 30 mins and measured after 90 mins of irradiation by Western blot analysis in presence of high NP-40 lysis buffer
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nucleophosmin (CHEMBL5178)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and validation of a novel assay to identify radiosensitizers that target nucleophosmin 1.
Penthala NR, Crooks PA, Freeman ML, Sekhar KR.
Bioorg Med Chem (2015) 23 : 3681 -3686
PubMed 25922180 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 1 5.60 5.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3589689 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3589689 EC50 = 2500.0 nM 5.60