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Assay Detail

CHEMBL3705295

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Fluorescence Polarization Competition Assay: An autophosphorylation, fluroescence polarization competition immunoassay was used to determine the potency for c-fms inhibition.
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Macrophage colony-stimulating factor 1 receptor (CHEMBL5570)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

4-Cyano-1H-imidazole-2-carboxylic acid [2-cyclohex-1-enyl-4-(2,6-dioxo-piperidin-4-yl)-phenyl]-amide; tyrosine kinase inhibitor; autoimmune diseases, antiinflammatory, anticarcinogenic agent
(2013)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 8.73 9.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3674575 1 9.39
CHEMBL3674563 1 9.38
CHEMBL3639734 1 9.37
CHEMBL3674560 1 9.33
CHEMBL3674558 1 9.19
CHEMBL3674579 1 9.18
CHEMBL3905759 1 9.18
CHEMBL3674559 1 9.14
CHEMBL3919523 1 9.14
CHEMBL3986577 1 9.09
CHEMBL3956660 1 9.07
CHEMBL3674569 1 8.96
CHEMBL3963729 1 8.96
CHEMBL3674571 1 8.85
CHEMBL3908207 1 8.74
CHEMBL3674562 1 8.70
CHEMBL3674577 1 8.70
CHEMBL3674561 1 8.57
CHEMBL3674570 EDICOTINIB 2.0 1 8.54
CHEMBL3968152 1 8.54
CHEMBL3674565 1 8.46
CHEMBL3956906 1 8.34
CHEMBL3674578 1 8.15
CHEMBL3674576 1 8.05
CHEMBL3674581 1 7.85
CHEMBL3674580 1 7.80
CHEMBL3674582 1 7.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3674575 IC50 = 0.41 nM 9.39
CHEMBL3674563 IC50 = 0.42 nM 9.38
CHEMBL3639734 IC50 = 0.43 nM 9.37
CHEMBL3674560 IC50 = 0.47 nM 9.33
CHEMBL3674558 IC50 = 0.65 nM 9.19
CHEMBL3674579 IC50 = 0.66 nM 9.18
CHEMBL3905759 IC50 = 0.66 nM 9.18
CHEMBL3674559 IC50 = 0.72 nM 9.14
CHEMBL3919523 IC50 = 0.72 nM 9.14
CHEMBL3986577 IC50 = 0.81 nM 9.09
CHEMBL3956660 IC50 = 0.86 nM 9.07
CHEMBL3674569 IC50 = 1.1 nM 8.96
CHEMBL3963729 IC50 = 1.1 nM 8.96
CHEMBL3674571 IC50 = 1.4 nM 8.85
CHEMBL3908207 IC50 = 1.8 nM 8.74
CHEMBL3674562 IC50 = 2.0 nM 8.70
CHEMBL3674577 IC50 = 2.0 nM 8.70
CHEMBL3674561 IC50 = 2.7 nM 8.57
CHEMBL3674570 EDICOTINIB IC50 = 2.9 nM 8.54
CHEMBL3968152 IC50 = 2.9 nM 8.54
CHEMBL3674565 IC50 = 3.5 nM 8.46
CHEMBL3956906 IC50 = 4.6 nM 8.34
CHEMBL3674578 IC50 = 7.1 nM 8.15
CHEMBL3674576 IC50 = 8.9 nM 8.05
CHEMBL3674581 IC50 = 14.0 nM 7.85
CHEMBL3674580 IC50 = 16.0 nM 7.80
CHEMBL3674582 IC50 = 99.0 nM 7.00