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Assay Detail

CHEMBL3705624

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition Assay: Inhibition of human FIIa was determined by the method described in [0092]-[0098] using human alpha-thrombin from Enzyme Research Laboratories at 0.1 NIH U/mL and Mes-d-Cha-Gly-Arg-pNA (Pefachrome tPA) at 2 mM, 1 mM, and 0.5 mM as substrate; results are reported as Ki values (nanomolar).
23
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Transcription initiation factor IIA subunit 1 (CHEMBL4832)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Trypsin-like serine protease inhibitors, and their preparation and use
(2013)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 23 5.36 6.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3660186 1 6.62
CHEMBL3660173 1 6.10
CHEMBL3660191 1 5.92
CHEMBL3660187 1 5.77
CHEMBL3660189 1 5.44
CHEMBL3660176 1 5.42
CHEMBL3660179 1 5.41
CHEMBL3660177 1 5.38
CHEMBL3660171 1 5.36
CHEMBL3660178 1 5.35
CHEMBL3660192 1 5.33
CHEMBL3660181 1 5.32
CHEMBL3660184 2 5.31
CHEMBL3660182 1 5.26
CHEMBL3660175 1 5.25
CHEMBL3660172 1 5.10
CHEMBL3660174 1 5.07
CHEMBL3660188 1 5.05
CHEMBL3660180 1 4.89
CHEMBL3660183 1 4.89
CHEMBL3660185 2 4.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3660186 Ki = 240.0 nM 6.62
CHEMBL3660173 Ki = 800.0 nM 6.10
CHEMBL3660191 Ki = 1200.0 nM 5.92
CHEMBL3660187 Ki = 1700.0 nM 5.77
CHEMBL3660189 Ki = 3600.0 nM 5.44
CHEMBL3660176 Ki = 3800.0 nM 5.42
CHEMBL3660179 Ki = 3900.0 nM 5.41
CHEMBL3660177 Ki = 4200.0 nM 5.38
CHEMBL3660171 Ki = 4400.0 nM 5.36
CHEMBL3660178 Ki = 4500.0 nM 5.35
CHEMBL3660192 Ki = 4700.0 nM 5.33
CHEMBL3660181 Ki = 4800.0 nM 5.32
CHEMBL3660184 Ki = 4900.0 nM 5.31
CHEMBL3660184 Ki = 4900.0 nM 5.31
CHEMBL3660182 Ki = 5500.0 nM 5.26
CHEMBL3660175 Ki = 5600.0 nM 5.25
CHEMBL3660172 Ki = 8000.0 nM 5.10
CHEMBL3660174 Ki = 8500.0 nM 5.07
CHEMBL3660188 Ki = 9000.0 nM 5.05
CHEMBL3660185 Ki = 13000.0 nM 4.89
CHEMBL3660183 Ki = 13000.0 nM 4.89
CHEMBL3660185 Ki = 13000.0 nM 4.89
CHEMBL3660180 Ki = 13000.0 nM 4.89