Assay Detail
Binding
CHEMBL3706168
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Flashplate Assay: In vitro enzyme inhibition using scintillation of incorporated radio label (flashplate assay). Test compounds diluted in DMSO are mixed with a suitable substrate / co-substrate (here: bio- tinylated a-casein and 3P-ATP) in a corresponding assay buffer. Addition of the enzyme of interest (here: ALKl kinase) starts the enzyme reaction. The enzyme-catalyzed incorporation of radio label into the substrate is measured via scintillation. Incorporated radio label is separated from free radio label via specific binding of the biotinylated substrate to strepavidin-coated microliter plates (flashplates) and concomitant washing steps. The scintillation signal intensity (counts per minutes, cpm) is proportional to the enzyme activity. Enzyme inhibition results in a decreased signal intensity. IC50 values of the test compounds are determined by cpm-versus-[I] plots. Reaction buffer: Reaction buffer contains 50 mM Tris pi I 8.0 (Sigma), 1 mM MnCi2 (Sigma), 0.01% Nonidet P40 (Fluka).
38
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Hydroxymethylaryl-substituted pyrrolotriazines as ALK1 inhibitors
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 38 | 8.14 | 9.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3667990 | — | — | 1 | 9.00 |
| CHEMBL3667966 | — | — | 1 | 9.00 |
| CHEMBL3667999 | — | — | 3 | 8.92 |
| CHEMBL3667967 | — | — | 1 | 8.89 |
| CHEMBL3668000 | — | — | 1 | 8.72 |
| CHEMBL3667973 | — | — | 1 | 8.72 |
| CHEMBL3667963 | — | — | 1 | 8.70 |
| CHEMBL3667969 | — | — | 1 | 8.70 |
| CHEMBL3667991 | — | — | 1 | 8.70 |
| CHEMBL3667979 | — | — | 1 | 8.70 |
| CHEMBL3667978 | — | — | 1 | 8.59 |
| CHEMBL3667964 | — | — | 1 | 8.55 |
| CHEMBL3668001 | — | — | 1 | 8.42 |
| CHEMBL3667993 | — | — | 1 | 8.40 |
| CHEMBL3667992 | — | — | 1 | 8.40 |
| CHEMBL3667975 | — | — | 1 | 8.40 |
| CHEMBL3667965 | — | — | 1 | 8.40 |
| CHEMBL3667995 | — | — | 1 | 8.39 |
| CHEMBL3667970 | — | — | 1 | 8.36 |
| CHEMBL3667976 | — | — | 1 | 8.28 |
| CHEMBL3667971 | — | — | 1 | 8.24 |
| CHEMBL3667974 | — | — | 1 | 8.23 |
| CHEMBL3667972 | — | — | 1 | 8.10 |
| CHEMBL3667994 | — | — | 1 | 7.82 |
| CHEMBL3667983 | — | — | 1 | 7.77 |
| CHEMBL3667981 | — | — | 1 | 7.72 |
| CHEMBL3667987 | — | — | 1 | 7.52 |
| CHEMBL3667984 | — | — | 1 | 7.52 |
| CHEMBL3667982 | — | — | 1 | 7.52 |
| CHEMBL3667968 | — | — | 1 | 7.40 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3667990 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3667966 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL3667999 | — | IC50 | = | 1.2 | nM | 8.92 |
| CHEMBL3667967 | — | IC50 | = | 1.3 | nM | 8.89 |
| CHEMBL3668000 | — | IC50 | = | 1.9 | nM | 8.72 |
| CHEMBL3667973 | — | IC50 | = | 1.9 | nM | 8.72 |
| CHEMBL3667963 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3667969 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3667991 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3667979 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL3667978 | — | IC50 | = | 2.6 | nM | 8.59 |
| CHEMBL3667964 | — | IC50 | = | 2.8 | nM | 8.55 |
| CHEMBL3667999 | — | IC50 | = | 3.2 | nM | 8.49 |
| CHEMBL3667999 | — | IC50 | = | 3.5 | nM | 8.46 |
| CHEMBL3668001 | — | IC50 | = | 3.8 | nM | 8.42 |
| CHEMBL3667965 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3667993 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3667992 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3667975 | — | IC50 | = | 4.0 | nM | 8.40 |
| CHEMBL3667995 | — | IC50 | = | 4.1 | nM | 8.39 |
| CHEMBL3667970 | — | IC50 | = | 4.4 | nM | 8.36 |
| CHEMBL3667976 | — | IC50 | = | 5.3 | nM | 8.28 |
| CHEMBL3667971 | — | IC50 | = | 5.7 | nM | 8.24 |
| CHEMBL3667974 | — | IC50 | = | 5.9 | nM | 8.23 |
| CHEMBL3667972 | — | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL3667994 | — | IC50 | = | 15.0 | nM | 7.82 |
| CHEMBL3667983 | — | IC50 | = | 17.0 | nM | 7.77 |
| CHEMBL3667981 | — | IC50 | = | 19.0 | nM | 7.72 |
| CHEMBL3667984 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3667982 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3667987 | — | IC50 | = | 30.0 | nM | 7.52 |
| CHEMBL3667968 | — | IC50 | = | 40.0 | nM | 7.40 |
| CHEMBL3667977 | — | IC50 | = | 60.0 | nM | 7.22 |
| CHEMBL3667986 | — | IC50 | = | 65.0 | nM | 7.19 |
| CHEMBL3667988 | — | IC50 | = | 70.0 | nM | 7.16 |
| CHEMBL3667980 | — | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL3667985 | — | IC50 | = | 140.0 | nM | 6.85 |
| CHEMBL3667989 | — | IC50 | = | 170.0 | nM | 6.77 |