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Assay Detail

CHEMBL3706359

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Scintillation Proximity Assay (SPA): The kinase assay is based on the LanthaScreen technology. LanthaScreen is the detection of Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) using lanthanide chelates to measure interactions between various binding partners. In a TR-FRET kinase assay, a long-lifetime lanthanide donor species is conjugated to an antibody that specifically binds to a phosphorylated product of a kinase reaction that is labeled with a suitable acceptor fluorophore. This antibody-mediated interaction brings the lanthanide donor and the acceptor into proximity such that resonance energy transfer can take place, resulting in a detectable increase in the FRET signal.The kinase reactions were performed in 384 well microtiter plates in a total reaction volume of 10.05 μL. The assay plates were prepared with 0.05 μL per well of test compound in the appropriate test concentration, as described under preparation of compound dilutions.
51
Total Activities
51
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Phosphodiesterase 3 (CHEMBL2094125)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Triazolopyridine compounds
(2015)

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 51 4.81 5.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1236107 SGX-523 1.0 1 5.52
CHEMBL3651404 1 5.40
CHEMBL2133806 JNJ-38877605 1.0 1 5.40
CHEMBL3704015 1 5.22
CHEMBL3703994 1 5.22
CHEMBL2001019 PF-04217903 1.0 1 5.16
CHEMBL3704020 1 5.16
CHEMBL3703986 1 5.16
CHEMBL3703992 1 5.00
CHEMBL3704000 1 5.00
CHEMBL3704013 1 4.96
CHEMBL3704008 1 4.92
CHEMBL3703996 1 4.92
CHEMBL3703982 1 4.92
CHEMBL3703989 1 4.89
CHEMBL3703993 1 4.89
CHEMBL3703940 1 4.85
CHEMBL3704007 1 4.85
CHEMBL3703988 1 4.85
CHEMBL3703957 1 4.82
CHEMBL3704018 1 4.82
CHEMBL3703970 1 4.80
CHEMBL3703961 1 4.77
CHEMBL3703947 1 4.77
CHEMBL3703991 1 4.77
CHEMBL3703997 1 4.75
CHEMBL3704005 1 4.75
CHEMBL3703979 1 4.70
CHEMBL3704014 1 4.70
CHEMBL3703950 1 4.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1236107 SGX-523 IC50 = 3000.0 nM 5.52
CHEMBL3651404 IC50 = 4000.0 nM 5.40
CHEMBL2133806 JNJ-38877605 IC50 = 4000.0 nM 5.40
CHEMBL3704015 IC50 = 6000.0 nM 5.22
CHEMBL3703994 IC50 = 6000.0 nM 5.22
CHEMBL2001019 PF-04217903 IC50 = 7000.0 nM 5.16
CHEMBL3704020 IC50 = 7000.0 nM 5.16
CHEMBL3703986 IC50 = 7000.0 nM 5.16
CHEMBL3703992 IC50 = 10000.0 nM 5.00
CHEMBL3704000 IC50 = 10000.0 nM 5.00
CHEMBL3704013 IC50 = 11000.0 nM 4.96
CHEMBL3704008 IC50 = 12000.0 nM 4.92
CHEMBL3703996 IC50 = 12000.0 nM 4.92
CHEMBL3703982 IC50 = 12000.0 nM 4.92
CHEMBL3703993 IC50 = 13000.0 nM 4.89
CHEMBL3703989 IC50 = 13000.0 nM 4.89
CHEMBL3703940 IC50 = 14000.0 nM 4.85
CHEMBL3704007 IC50 = 14000.0 nM 4.85
CHEMBL3703988 IC50 = 14000.0 nM 4.85
CHEMBL3703957 IC50 = 15000.0 nM 4.82
CHEMBL3704018 IC50 = 15000.0 nM 4.82
CHEMBL3703970 IC50 = 16000.0 nM 4.80
CHEMBL3703961 IC50 = 17000.0 nM 4.77
CHEMBL3703947 IC50 = 17000.0 nM 4.77
CHEMBL3703991 IC50 = 17000.0 nM 4.77
CHEMBL3703997 IC50 = 18000.0 nM 4.75
CHEMBL3704005 IC50 = 18000.0 nM 4.75
CHEMBL3703979 IC50 = 20000.0 nM 4.70
CHEMBL3704014 IC50 = 20000.0 nM 4.70
CHEMBL3703950 IC50 = 24000.0 nM 4.62
CHEMBL3703964 IC50 = 25000.0 nM 4.60
CHEMBL3703966 IC50 = 26000.0 nM 4.58
CHEMBL3704017 IC50 = 27000.0 nM 4.57
CHEMBL3704009 IC50 = 28000.0 nM 4.55
CHEMBL3703978 IC50 = 29000.0 nM 4.54
CHEMBL3703945 IC50 = 30000.0 nM 4.52
CHEMBL3703965 IC50 = 30000.0 nM 4.52
CHEMBL3703968 IC50 = 30000.0 nM 4.52
CHEMBL3703981 IC50 = 30000.0 nM 4.52
CHEMBL3703985 IC50 = 30000.0 nM 4.52
CHEMBL3703958 IC50 = 30000.0 nM 4.52
CHEMBL3704011 IC50 = 30000.0 nM 4.52
CHEMBL3703956 IC50 = 30000.0 nM 4.52
CHEMBL3704016 IC50 = 30000.0 nM 4.52
CHEMBL3703952 IC50 = 30000.0 nM 4.52
CHEMBL3703943 IC50 = 30000.0 nM 4.52
CHEMBL3704010 IC50 > 30000.0 nM -
CHEMBL3704002 IC50 > 30000.0 nM -
CHEMBL3704001 IC50 > 30000.0 nM -
CHEMBL3704019 IC50 > 30000.0 nM -