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Assay Detail

CHEMBL3745570

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 2-[125I]-iodomelatonin from human melatonin receptor MT1 expressed in CHO cells after 1 hr by gamma counting analysis
23
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melatonin receptor type 1A (CHEMBL1945)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel difluoroacetamide analogues of agomelatine and melatonin: probing the melatonin receptors for MT1selectivity
Zlotos DP, Riad NM, Osman MB, Dodda BR, Witt-Enderby PA
Medchemcomm (2015) 6 : 1340 -1344
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 23 8.25 10.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1946221 2 10.27
CHEMBL45 MELATONIN 4.0 1 9.34
CHEMBL3741890 2 8.92
CHEMBL322339 2 8.30
CHEMBL3742090 2 8.30
CHEMBL3739674 2 8.21
CHEMBL3260986 2 8.10
CHEMBL3739824 2 8.05
CHEMBL3741561 2 8.05
CHEMBL3740377 2 7.93
CHEMBL3740052 2 7.46
CHEMBL429392 2 6.95

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1946221 Ki = 0.054 nM 10.27
CHEMBL1946221 Ki = 0.0537 nM 10.27
CHEMBL45 MELATONIN Ki = 0.4571 nM 9.34
CHEMBL3741890 Ki = 1.2 nM 8.92
CHEMBL3741890 Ki = 1.23 nM 8.91
CHEMBL3742090 Ki = 5.012 nM 8.30
CHEMBL3742090 Ki = 5.0 nM 8.30
CHEMBL322339 Ki = 5.0 nM 8.30
CHEMBL322339 Ki = 5.754 nM 8.24
CHEMBL3739674 Ki = 6.2 nM 8.21
CHEMBL3739674 Ki = 6.166 nM 8.21
CHEMBL3260986 Ki = 7.943 nM 8.10
CHEMBL3260986 Ki = 7.9 nM 8.10
CHEMBL3741561 Ki = 9.0 nM 8.05
CHEMBL3739824 Ki = 9.0 nM 8.05
CHEMBL3741561 Ki = 9.333 nM 8.03
CHEMBL3740377 Ki = 11.75 nM 7.93
CHEMBL3740377 Ki = 12.0 nM 7.92
CHEMBL3739824 Ki = 25.12 nM 7.60
CHEMBL3740052 Ki = 35.0 nM 7.46
CHEMBL3740052 Ki = 35.48 nM 7.45
CHEMBL429392 Ki = 112.0 nM 6.95
CHEMBL429392 Ki = 112.2 nM 6.95