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Assay Detail

CHEMBL3750088

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MEK1 (unknown origin) using biotinylated ERK1 as substrate incubated for 2 hrs by fluorescence analysis
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Optimization of allosteric MEK inhibitors. Part 2: Taming the sulfamide group balances compound distribution properties.
Hartung IV, Hammer S, Hitchcock M, Neuhaus R, Scholz A, Siemeister G, Bohlmann R, Hillig RC, Pühler F.
Bioorg Med Chem Lett (2016) 26 : 186 -193
PubMed 26611920 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 7.29 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3747294 1 8.00
CHEMBL3746569 1 7.96
CHEMBL3747066 1 7.92
CHEMBL507361 MIRDAMETINIB 2.0 1 7.89
CHEMBL3746736 1 7.89
CHEMBL2376666 1 7.85
CHEMBL3747047 1 7.85
CHEMBL3746559 1 7.85
CHEMBL3747136 1 7.82
CHEMBL3746467 1 7.82
CHEMBL3747256 1 7.82
CHEMBL3747359 1 7.75
CHEMBL3746640 1 7.68
CHEMBL2376663 1 7.68
CHEMBL3746031 1 7.66
CHEMBL3746103 1 7.55
CHEMBL1614701 SELUMETINIB 4.0 1 7.51
CHEMBL2376667 1 7.50
CHEMBL3746537 1 7.50
CHEMBL3746923 1 7.43
CHEMBL3746142 1 7.09
CHEMBL3747516 1 7.08
CHEMBL3747368 1 7.03
CHEMBL3746158 1 6.97
CHEMBL3746516 1 6.93
CHEMBL3746203 1 6.90
CHEMBL3747610 1 6.58
CHEMBL3745994 1 6.46
CHEMBL3747708 1 6.42
CHEMBL3747637 1 6.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3747294 IC50 = 10.0 nM 8.00
CHEMBL3746569 IC50 = 11.0 nM 7.96
CHEMBL3747066 IC50 = 12.0 nM 7.92
CHEMBL507361 MIRDAMETINIB IC50 = 13.0 nM 7.89
CHEMBL3746736 IC50 = 13.0 nM 7.89
CHEMBL2376666 IC50 = 14.0 nM 7.85
CHEMBL3747047 IC50 = 14.0 nM 7.85
CHEMBL3746559 IC50 = 14.0 nM 7.85
CHEMBL3747136 IC50 = 15.0 nM 7.82
CHEMBL3746467 IC50 = 15.0 nM 7.82
CHEMBL3747256 IC50 = 15.0 nM 7.82
CHEMBL3747359 IC50 = 18.0 nM 7.75
CHEMBL3746640 IC50 = 21.0 nM 7.68
CHEMBL2376663 IC50 = 21.0 nM 7.68
CHEMBL3746031 IC50 = 22.0 nM 7.66
CHEMBL3746103 IC50 = 28.0 nM 7.55
CHEMBL1614701 SELUMETINIB IC50 = 31.0 nM 7.51
CHEMBL2376667 IC50 = 32.0 nM 7.50
CHEMBL3746537 IC50 = 32.0 nM 7.50
CHEMBL3746923 IC50 = 37.0 nM 7.43
CHEMBL3746142 IC50 = 81.0 nM 7.09
CHEMBL3747516 IC50 = 83.0 nM 7.08
CHEMBL3747368 IC50 = 94.0 nM 7.03
CHEMBL3746158 IC50 = 107.0 nM 6.97
CHEMBL3746516 IC50 = 118.0 nM 6.93
CHEMBL3746203 IC50 = 127.0 nM 6.90
CHEMBL3747610 IC50 = 262.0 nM 6.58
CHEMBL3745994 IC50 = 345.0 nM 6.46
CHEMBL3747708 IC50 = 379.0 nM 6.42
CHEMBL3747637 IC50 = 592.0 nM 6.23
CHEMBL3747496 IC50 = 899.0 nM 6.05
CHEMBL3746690 IC50 = 941.0 nM 6.03
CHEMBL3746213 IC50 = 1900.0 nM 5.72