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Assay Detail

CHEMBL3768666

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length human C-terminal HA-tagged DAGLalpha expressed in HEK293 cell membrane using DAG as substrate incubated for 20 mins by LC-MS analysis
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Diacylglycerol lipase-alpha (CHEMBL5545)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure activity relationship studies on chemically non-reactive glycine sulfonamide inhibitors of diacylglycerol lipase.
Chupak LS, Zheng X, Hu S, Huang Y, Ding M, Lewis MA, Westphal RS, Blat Y, McClure A, Gentles RG.
Bioorg Med Chem (2016) 24 : 1455 -1468
PubMed 26917221 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.52 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3764017 1 9.22
CHEMBL3765578 1 9.05
CHEMBL3764876 1 8.62
CHEMBL3763826 1 8.52
CHEMBL3765716 1 8.52
CHEMBL3763853 1 8.52
CHEMBL3763831 1 8.40
CHEMBL3764093 1 8.22
CHEMBL3765421 1 8.22
CHEMBL3765022 1 8.10
CHEMBL3763430 1 8.10
CHEMBL3765158 1 7.96
CHEMBL3765824 1 7.80
CHEMBL3764778 1 7.77
CHEMBL3764377 1 7.77
CHEMBL3765573 1 7.75
CHEMBL3765497 1 7.72
CHEMBL3765546 1 7.52
CHEMBL3764349 1 7.40
CHEMBL3765562 1 7.35
CHEMBL3764044 1 7.26
CHEMBL3763964 1 7.22
CHEMBL3765750 1 7.10
CHEMBL3765634 1 7.10
CHEMBL3764007 1 6.89
CHEMBL3764430 1 6.79
CHEMBL3763708 1 6.29
CHEMBL3763737 1 4.87
CHEMBL3764425 1 4.85
CHEMBL3763376 1 4.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3764017 IC50 = 0.6 nM 9.22
CHEMBL3765578 IC50 = 0.9 nM 9.05
CHEMBL3764876 IC50 = 2.4 nM 8.62
CHEMBL3763826 IC50 = 3.0 nM 8.52
CHEMBL3765716 IC50 = 3.0 nM 8.52
CHEMBL3763853 IC50 = 3.0 nM 8.52
CHEMBL3763831 IC50 = 4.0 nM 8.40
CHEMBL3764093 IC50 = 6.0 nM 8.22
CHEMBL3765421 IC50 = 6.0 nM 8.22
CHEMBL3765022 IC50 = 8.0 nM 8.10
CHEMBL3763430 IC50 = 8.0 nM 8.10
CHEMBL3765158 IC50 = 11.0 nM 7.96
CHEMBL3765824 IC50 = 16.0 nM 7.80
CHEMBL3764778 IC50 = 17.0 nM 7.77
CHEMBL3764377 IC50 = 17.0 nM 7.77
CHEMBL3765573 IC50 = 18.0 nM 7.75
CHEMBL3765497 IC50 = 19.0 nM 7.72
CHEMBL3765546 IC50 = 30.0 nM 7.52
CHEMBL3764349 IC50 = 40.0 nM 7.40
CHEMBL3765562 IC50 = 45.0 nM 7.35
CHEMBL3764044 IC50 = 55.0 nM 7.26
CHEMBL3763964 IC50 = 60.0 nM 7.22
CHEMBL3765750 IC50 = 80.0 nM 7.10
CHEMBL3765634 IC50 = 80.0 nM 7.10
CHEMBL3764007 IC50 = 130.0 nM 6.89
CHEMBL3764430 IC50 = 161.0 nM 6.79
CHEMBL3763708 IC50 = 510.0 nM 6.29
CHEMBL3763737 IC50 = 13500.0 nM 4.87
CHEMBL3764425 IC50 = 14000.0 nM 4.85
CHEMBL3763376 IC50 = 16000.0 nM 4.80
CHEMBL3764769 IC50 > 30000.0 nM -