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Assay Detail

CHEMBL3796544

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Binding
Agonist activity at human TGR5 expressed in CHO-K1 cells after 5 hrs by luciferase reporter gene assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled bile acid receptor 1 (CHEMBL5409)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Potent and Orally Efficacious TGR5 Receptor Agonist.
Agarwal S, Patil A, Aware U, Deshmukh P, Darji B, Sasane S, Sairam KV, Priyadarsiny P, Giri P, Patel H, Giri S, Jain M, Desai RC.
ACS Med Chem Lett (2016) 7 : 51 -55
PubMed 26819665 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 8.21 10.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3793436 1 10.24
CHEMBL3794442 1 8.83
CHEMBL3793012 1 8.64
CHEMBL3793388 1 8.24
CHEMBL3792957 1 8.11
CHEMBL3793168 1 8.07
CHEMBL3794111 1 7.78
CHEMBL3794175 1 7.77
CHEMBL3793859 1 7.71
CHEMBL3794424 1 7.50
CHEMBL3794293 1 7.42
CHEMBL3794367 1 -
CHEMBL3792938 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3793436 EC50 = 0.057 nM 10.24
CHEMBL3794442 EC50 = 1.48 nM 8.83
CHEMBL3793012 EC50 = 2.3 nM 8.64
CHEMBL3793388 EC50 = 5.8 nM 8.24
CHEMBL3792957 EC50 = 7.8 nM 8.11
CHEMBL3793168 EC50 = 8.6 nM 8.07
CHEMBL3794111 EC50 = 16.8 nM 7.78
CHEMBL3794175 EC50 = 17.08 nM 7.77
CHEMBL3793859 EC50 = 19.6 nM 7.71
CHEMBL3794424 EC50 = 31.5 nM 7.50
CHEMBL3794293 EC50 = 38.0 nM 7.42
CHEMBL3794367 EC50 > 100.0 nM -
CHEMBL3792938 EC50 > 100.0 nM -