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Assay Detail

CHEMBL3800925

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SERT W103A mutant expressed in HEK293 MSR cells assessed as [3H]-5-HT uptake preincubated for 30 mins followed addition of [3H]-5HT and compound for 10 mins by micro-scintillation counter
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent serotonin transporter (CHEMBL228)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and inhibitory evaluation of 3-linked imipramines for the exploration of the S2 site of the human serotonin transporter.
Brinkø A, Larsen MT, Koldsø H, Besenbacher L, Kolind A, Schiøtt B, Sinning S, Jensen HH.
Bioorg Med Chem (2016) 24 : 2725 -2738
PubMed 27160055 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 6.44 8.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL11 IMIPRAMINE 4.0 1 8.59
CHEMBL3798035 1 7.40
CHEMBL3797621 1 7.22
CHEMBL3799356 1 6.57
CHEMBL3799596 1 6.55
CHEMBL3799668 1 6.55
CHEMBL3799795 1 6.52
CHEMBL3797849 1 6.47
CHEMBL3799503 1 6.46
CHEMBL3798279 1 6.38
CHEMBL3799072 1 6.36
CHEMBL3799481 1 6.25
CHEMBL3799640 1 6.22
CHEMBL3799416 1 6.21
CHEMBL3799319 1 6.08
CHEMBL3799011 1 6.03
CHEMBL3799131 1 5.80
CHEMBL3800215 1 5.39
CHEMBL3798819 1 5.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL11 IMIPRAMINE IC50 = 2.6 nM 8.59
CHEMBL3798035 IC50 = 40.0 nM 7.40
CHEMBL3797621 IC50 = 60.0 nM 7.22
CHEMBL3799356 IC50 = 270.0 nM 6.57
CHEMBL3799596 IC50 = 280.0 nM 6.55
CHEMBL3799668 IC50 = 280.0 nM 6.55
CHEMBL3799795 IC50 = 300.0 nM 6.52
CHEMBL3797849 IC50 = 340.0 nM 6.47
CHEMBL3799503 IC50 = 350.0 nM 6.46
CHEMBL3798279 IC50 = 420.0 nM 6.38
CHEMBL3799072 IC50 = 440.0 nM 6.36
CHEMBL3799481 IC50 = 560.0 nM 6.25
CHEMBL3799640 IC50 = 600.0 nM 6.22
CHEMBL3799416 IC50 = 610.0 nM 6.21
CHEMBL3799319 IC50 = 840.0 nM 6.08
CHEMBL3799011 IC50 = 940.0 nM 6.03
CHEMBL3799131 IC50 = 1600.0 nM 5.80
CHEMBL3800215 IC50 = 4100.0 nM 5.39
CHEMBL3798819 IC50 = 4800.0 nM 5.32