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Assay Detail

CHEMBL3821695

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP2C9 in human liver microsomes using tolbutamide as substrate after 20 mins by LC-MS analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 2C9 (CHEMBL3397)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Understanding Oxadiazolothiazinone Biological Properties: Negative Inotropic Activity versus Cytochrome P450-Mediated Metabolism.
Carosati E, Cosimelli B, Ioan P, Severi E, Katneni K, Chiu FC, Saponara S, Fusi F, Frosini M, Matucci R, Micucci M, Chiarini A, Spinelli D, Budriesi R.
J Med Chem (2016) 59 : 3340 -3352
PubMed 26962886 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.04 6.13

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1109 SULFAPHENAZOLE 4.0 1 6.13
CHEMBL3408523 1 5.18
CHEMBL3818560 1 4.93
CHEMBL3818643 1 4.87
CHEMBL3819606 1 4.74
CHEMBL211204 1 4.74
CHEMBL3819226 1 4.72
CHEMBL3819185 1 -
CHEMBL3819349 1 -
CHEMBL3408526 1 -
CHEMBL3408529 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1109 SULFAPHENAZOLE IC50 = 740.0 nM 6.13
CHEMBL3408523 IC50 = 6600.0 nM 5.18
CHEMBL3818560 IC50 = 11800.0 nM 4.93
CHEMBL3818643 IC50 = 13600.0 nM 4.87
CHEMBL3819606 IC50 = 18400.0 nM 4.74
CHEMBL211204 IC50 = 18400.0 nM 4.74
CHEMBL3819226 IC50 = 19000.0 nM 4.72
CHEMBL3819185 IC50 > 20000.0 nM -
CHEMBL3819349 IC50 > 20000.0 nM -
CHEMBL3408526 IC50 > 20000.0 nM -
CHEMBL3408529 IC50 > 20000.0 nM -