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Assay Detail

CHEMBL3821818

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SERT expressed in CHO cells assessed as [3H]-5-hydroxytryptamine reuptake incubated for 20 mins measured after 30 mins by topcount method
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent serotonin transporter (CHEMBL228)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and biological evaluation of a novel series of peripheral-selective noradrenaline reuptake inhibitors - Part 3.
Yukawa T, Nakada Y, Sakauchi N, Kamei T, Yamada M, Ohba Y, Fujimori I, Ueno H, Takiguchi M, Kuno M, Kamo I, Nakagawa H, Fujioka Y, Igari T, Ishichi Y, Tsukamoto T.
Bioorg Med Chem (2016) 24 : 3716 -3726
PubMed 27325446 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.05 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3818117 1 7.92
CHEMBL3817915 1 7.57
CHEMBL3818901 1 7.27
CHEMBL3818471 1 7.17
CHEMBL3819243 1 7.10
CHEMBL3818953 1 7.07
CHEMBL3819009 1 6.92
CHEMBL3819201 1 6.89
CHEMBL3818345 1 6.42
CHEMBL3818946 1 6.14
CHEMBL3593402 1 -
CHEMBL3819441 1 -
CHEMBL3818596 1 -
CHEMBL3819217 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3818117 IC50 = 12.0 nM 7.92
CHEMBL3817915 IC50 = 27.0 nM 7.57
CHEMBL3818901 IC50 = 54.0 nM 7.27
CHEMBL3818471 IC50 = 67.0 nM 7.17
CHEMBL3819243 IC50 = 79.0 nM 7.10
CHEMBL3818953 IC50 = 85.0 nM 7.07
CHEMBL3819009 IC50 = 120.0 nM 6.92
CHEMBL3819201 IC50 = 130.0 nM 6.89
CHEMBL3818345 IC50 = 380.0 nM 6.42
CHEMBL3818946 IC50 = 730.0 nM 6.14
CHEMBL3593402 IC50 > 1000.0 nM -
CHEMBL3819441 IC50 > 1000.0 nM -
CHEMBL3818596 IC50 > 1000.0 nM -
CHEMBL3819217 IC50 > 1000.0 nM -