Assay Detail
Binding
CHEMBL3829309
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Inhibition of equine serum BChE preincubated for 15 mins followed by addition of S-butyrylthiocholine chloride as substrate measured after 30 mins by Ellman's microplate assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Equus caballus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Cholinesterase inhibitory activity of isoquinoline alkaloids from three Cryptocarya species (Lauraceae).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 4.91 | 6.24 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL94 | PHYSOSTIGMINE | 3.0 | 1 | 6.24 |
| CHEMBL3827910 | — | — | 1 | 5.40 |
| CHEMBL1186488 | — | — | 1 | 4.71 |
| CHEMBL235212 | (S)-RETICULINE | — | 1 | 4.19 |
| CHEMBL3827872 | — | — | 1 | 4.03 |
| CHEMBL483227 | — | — | 1 | - |
| CHEMBL3827670 | — | — | 1 | - |
| CHEMBL464098 | LAUROTETANINE | — | 1 | - |
| CHEMBL3828751 | — | — | 1 | - |
| CHEMBL464099 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL94 | PHYSOSTIGMINE | IC50 | = | 580.0 | nM | 6.24 |
| CHEMBL3827910 | — | IC50 | = | 3950.0 | nM | 5.40 |
| CHEMBL1186488 | — | IC50 | = | 19340.0 | nM | 4.71 |
| CHEMBL235212 | (S)-RETICULINE | IC50 | = | 65040.0 | nM | 4.19 |
| CHEMBL3827872 | — | IC50 | = | 94450.0 | nM | 4.03 |
| CHEMBL483227 | — | IC50 | = | 166690.0 | nM | - |
| CHEMBL3827670 | — | IC50 | = | 288340.0 | nM | - |
| CHEMBL464098 | LAUROTETANINE | IC50 | - | — | - | |
| CHEMBL3828751 | — | IC50 | - | — | - | |
| CHEMBL464099 | — | IC50 | = | 218810.0 | nM | - |