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Assay Detail

CHEMBL3863952

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PDE4 in human whole blood assessed as reduction in LPS-induced TNFalpha production incubated for 24 hrs by ELISA
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Blood
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Biphenyl Pyridazinone Derivatives as Inhaled PDE4 Inhibitors: Structural Biology and Structure-Activity Relationships.
Gràcia J, Buil MA, Castro J, Eichhorn P, Ferrer M, Gavaldà A, Hernández B, Segarra V, Lehner MD, Moreno I, Pagès L, Roberts RS, Serrat J, Sevilla S, Taltavull J, Andrés M, Cabedo J, Vilella D, Calama E, Carcasona C, Miralpeix M.
J Med Chem (2016) 59 : 10479 -10497
PubMed 27933955 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 6.88 7.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 7.44
CHEMBL3968147 1 7.24
CHEMBL3986586 1 7.23
CHEMBL3903604 1 7.19
CHEMBL3958944 1 7.17
CHEMBL3952586 1 7.16
CHEMBL3918173 1 7.16
CHEMBL3958869 1 7.12
CHEMBL3941513 1 7.09
CHEMBL3920544 1 7.03
CHEMBL3969954 1 7.02
CHEMBL3957296 1 7.00
CHEMBL3889823 1 6.93
CHEMBL3925776 1 6.92
CHEMBL3916846 1 6.90
CHEMBL3974515 1 6.81
CHEMBL3950249 1 6.72
CHEMBL3979212 1 6.70
CHEMBL3950166 1 6.68
CHEMBL3892121 1 6.59
CHEMBL3957127 1 6.49
CHEMBL3905929 1 6.43
CHEMBL3984674 1 6.43
CHEMBL3980193 1 6.37
CHEMBL3896989 1 6.28
CHEMBL3898745 1 -
CHEMBL3978291 1 -
CHEMBL3912622 1 -
CHEMBL3966020 1 -
CHEMBL3935796 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST IC50 = 36.0 nM 7.44
CHEMBL3968147 IC50 = 57.0 nM 7.24
CHEMBL3986586 IC50 = 59.0 nM 7.23
CHEMBL3903604 IC50 = 64.0 nM 7.19
CHEMBL3958944 IC50 = 67.0 nM 7.17
CHEMBL3952586 IC50 = 69.0 nM 7.16
CHEMBL3918173 IC50 = 70.0 nM 7.16
CHEMBL3958869 IC50 = 75.0 nM 7.12
CHEMBL3941513 IC50 = 81.0 nM 7.09
CHEMBL3920544 IC50 = 93.0 nM 7.03
CHEMBL3969954 IC50 = 95.0 nM 7.02
CHEMBL3957296 IC50 = 100.0 nM 7.00
CHEMBL3889823 IC50 = 117.0 nM 6.93
CHEMBL3925776 IC50 = 120.0 nM 6.92
CHEMBL3916846 IC50 = 125.0 nM 6.90
CHEMBL3974515 IC50 = 154.0 nM 6.81
CHEMBL3950249 IC50 = 190.0 nM 6.72
CHEMBL3979212 IC50 = 202.0 nM 6.70
CHEMBL3950166 IC50 = 207.0 nM 6.68
CHEMBL3892121 IC50 = 257.0 nM 6.59
CHEMBL3957127 IC50 = 323.0 nM 6.49
CHEMBL3905929 IC50 = 374.0 nM 6.43
CHEMBL3984674 IC50 = 373.0 nM 6.43
CHEMBL3980193 IC50 = 425.0 nM 6.37
CHEMBL3896989 IC50 = 525.0 nM 6.28
CHEMBL3898745 IC50 - -
CHEMBL3978291 IC50 - -
CHEMBL3912622 IC50 - -
CHEMBL3966020 IC50 - -
CHEMBL3935796 IC50 - -
CHEMBL3909436 IC50 - -
CHEMBL3931354 IC50 - -
CHEMBL3963331 IC50 - -