Assay Detail
Binding
CHEMBL3866459
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human CDK1/cyclinA expressed in Baculovirus infected Sf9 cells using Biotin-aminohexyl-Ala-Arg-Arg-Pro-Met-Ser-Pro-Lys-LysLys-Ala-CONH2 as substrate measured after 30 to 60 mins in presence of [gamma-32P]ATP by scintillation counting method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Publication
3-Hydrazinoindolin-2-one derivatives: Chemical classification and investigation of their targets as anticancer agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 7.49 | 8.55 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL410883 | — | — | 1 | 8.55 |
| CHEMBL269827 | GW276655X | — | 1 | 8.00 |
| CHEMBL409892 | GW305178X | — | 1 | 7.92 |
| CHEMBL411426 | — | — | 1 | 7.43 |
| CHEMBL271160 | — | — | 1 | 7.39 |
| CHEMBL407709 | — | — | 1 | 7.37 |
| CHEMBL272387 | — | — | 1 | 6.77 |
| CHEMBL272389 | — | — | 1 | 6.46 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL410883 | — | IC50 | = | 2.8 | nM | 8.55 |
| CHEMBL269827 | GW276655X | IC50 | = | 10.0 | nM | 8.00 |
| CHEMBL409892 | GW305178X | IC50 | = | 12.0 | nM | 7.92 |
| CHEMBL411426 | — | IC50 | = | 37.0 | nM | 7.43 |
| CHEMBL271160 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL407709 | — | IC50 | = | 43.0 | nM | 7.37 |
| CHEMBL272387 | — | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL272389 | — | IC50 | = | 350.0 | nM | 6.46 |