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Assay Detail

CHEMBL3867192

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP3A4 in human liver microsomes using testosterone as substrate in presence of NADPH by LC-MS/MS analysis
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Minimizing CYP2C9 Inhibition of Exposed-Pyridine NAMPT (Nicotinamide Phosphoribosyltransferase) Inhibitors.
Zak M, Yuen PW, Liu X, Patel S, Sampath D, Oeh J, Liederer BM, Wang W, O'Brien T, Xiao Y, Skelton N, Hua R, Sodhi J, Wang Y, Zhang L, Zhao G, Zheng X, Ho YC, Bair KW, Dragovich PS.
J Med Chem (2016) 59 : 8345 -8368
PubMed 27541271 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.16 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3127521 1 7.05
CHEMBL17289 CHS-828 1.0 1 6.66
CHEMBL566757 DAPORINAD 2.0 1 5.82
CHEMBL3260358 1 5.10
CHEMBL2420629 1 -
CHEMBL2391568 1 -
CHEMBL2419515 1 -
CHEMBL3394738 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3127521 IC50 = 90.0 nM 7.05
CHEMBL17289 CHS-828 IC50 = 220.0 nM 6.66
CHEMBL566757 DAPORINAD IC50 = 1500.0 nM 5.82
CHEMBL3260358 IC50 = 7900.0 nM 5.10
CHEMBL2420629 IC50 > 10000.0 nM -
CHEMBL2391568 IC50 > 10000.0 nM -
CHEMBL2419515 IC50 > 10000.0 nM -
CHEMBL3394738 IC50 > 10000.0 nM -