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Assay Detail

CHEMBL3868882

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Wnt signalling in human STF/293 cells assessed as suppression of TCF/beta-catenin-mediated transcriptional activity after 24 hrs by superTOPflash reporter gene assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type STF/293
Confidence 3 — Cell-line / Tissue
Curated By Autocuration

Publication

Cerasoidine, a Bis-aporphine Alkaloid Isolated from Polyalthia cerasoides during Screening for Wnt Signal Inhibitors.
Shono T, Ishikawa N, Toume K, Arai MA, Masu H, Koyano T, Kowithayakorn T, Ishibashi M.
J Nat Prod (2016) 79 : 2083 -2088
PubMed 27490091 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.47 6.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL37736 LIRIODENINE 1 6.22
CHEMBL3950972 1 6.22
CHEMBL389400 ATHEROSPERMIDINE 1 5.15
CHEMBL3924309 1 5.06
CHEMBL400245 BIDEBILINE E 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL37736 LIRIODENINE IC50 = 600.0 nM 6.22
CHEMBL3950972 IC50 = 600.0 nM 6.22
CHEMBL389400 ATHEROSPERMIDINE IC50 = 7100.0 nM 5.15
CHEMBL3924309 IC50 = 8700.0 nM 5.06
CHEMBL400245 BIDEBILINE E IC50 = 20200.0 nM 4.70