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Assay Detail

CHEMBL3871875

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]citalopram from human SERT expressed in HEK293 cell membranes after 1 hr by liquid scintillation counting method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent serotonin transporter (CHEMBL228)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of SMP-304, a novel benzylpiperidine derivative with serotonin transporter inhibitory activity and 5-HT1A weak partial agonistic activity showing the antidepressant-like effect.
Yoshinaga H, Masumoto S, Koyama K, Kinomura N, Matsumoto Y, Kato T, Baba S, Matsumoto K, Horisawa T, Oki H, Yabuuchi K, Kodo T.
Bioorg Med Chem (2017) 25 : 293 -304
PubMed 27865645 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 7.67 9.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL490 PAROXETINE 4.0 1 9.96
CHEMBL11 IMIPRAMINE 4.0 1 8.70
CHEMBL259209 MILNACIPRAN 4.0 1 7.66
CHEMBL3913541 1 7.18
CHEMBL3894524 1 6.99
CHEMBL3966886 1 6.67
CHEMBL3892582 1 6.56
CHEMBL3981001 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL490 PAROXETINE Ki = 0.11 nM 9.96
CHEMBL11 IMIPRAMINE Ki = 2.0 nM 8.70
CHEMBL259209 MILNACIPRAN Ki = 22.0 nM 7.66
CHEMBL3913541 Ki = 66.4 nM 7.18
CHEMBL3894524 Ki = 102.0 nM 6.99
CHEMBL3966886 Ki = 212.0 nM 6.67
CHEMBL3892582 Ki = 274.0 nM 6.56
CHEMBL3981001 Ki < 100.0 nM -