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Assay Detail

CHEMBL4008248

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against GFP-fused Ebolavirus infected in TERT-immortalized HMVEC assessed as reduction in viral replication preincubated with cells followed by viral infection measured after 3 to 4 days by fluorescence assay
24
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Ebolavirus
Confidence 1 — Organism
Curated By Autocuration

Target

Ebolavirus (CHEMBL4434621)
Type ORGANISM
Organism Ebolavirus

Publication

Discovery and Synthesis of a Phosphoramidate Prodrug of a Pyrrolo[2,1-f][triazin-4-amino] Adenine C-Nucleoside (GS-5734) for the Treatment of Ebola and Emerging Viruses.
Siegel D, Hui HC, Doerffler E, Clarke MO, Chun K, Zhang L, Neville S, Carra E, Lew W, Ross B, Wang Q, Wolfe L, Jordan R, Soloveva V, Knox J, Perry J, Perron M, Stray KM, Barauskas O, Feng JY, Xu Y, Lee G, Rheingold AL, Ray AS, Bannister R, Strickley R, Swaminathan S, Lee WA, Bavari S, Cihlar T, Lo MK, Warren TK, Mackman RL.
J Med Chem (2017) 60 : 1648 -1661
PubMed 28124907 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 24 6.03 7.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4061258 1 7.28
CHEMBL4065616 REMDESIVIR 4.0 2 7.28
CHEMBL4065821 1 7.06
CHEMBL4083126 1 7.04
CHEMBL4072649 1 6.80
CHEMBL4064050 1 6.43
CHEMBL4090912 1 6.29
CHEMBL4084735 1 6.23
CHEMBL4093434 1 6.20
CHEMBL4098638 1 6.17
CHEMBL2016757 GS-441524 1.0 1 6.11
CHEMBL4099554 1 6.07
CHEMBL4074442 1 5.80
CHEMBL4101182 1 5.50
CHEMBL4091811 1 5.42
CHEMBL4086934 1 5.41
CHEMBL4069927 1 5.04
CHEMBL3120789 1 4.89
CHEMBL3120793 GS-6620 1.0 1 4.40
CHEMBL4065501 1 4.25
CHEMBL3120779 1 -
CHEMBL4090316 1 -
CHEMBL2016758 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4061258 EC50 = 53.0 nM 7.28
CHEMBL4065616 REMDESIVIR EC50 = 53.0 nM 7.28
CHEMBL4065821 EC50 = 88.0 nM 7.06
CHEMBL4083126 EC50 = 92.0 nM 7.04
CHEMBL4065616 REMDESIVIR EC50 = 120.0 nM 6.92
CHEMBL4072649 EC50 = 160.0 nM 6.80
CHEMBL4064050 EC50 = 367.0 nM 6.43
CHEMBL4090912 EC50 = 518.0 nM 6.29
CHEMBL4084735 EC50 = 587.0 nM 6.23
CHEMBL4093434 EC50 = 636.0 nM 6.20
CHEMBL4098638 EC50 = 678.0 nM 6.17
CHEMBL2016757 GS-441524 EC50 = 780.0 nM 6.11
CHEMBL4099554 EC50 = 845.0 nM 6.07
CHEMBL4074442 EC50 = 1585.0 nM 5.80
CHEMBL4101182 EC50 = 3151.0 nM 5.50
CHEMBL4091811 EC50 = 3790.0 nM 5.42
CHEMBL4086934 EC50 = 3900.0 nM 5.41
CHEMBL4069927 EC50 = 9102.0 nM 5.04
CHEMBL3120789 EC50 = 13000.0 nM 4.89
CHEMBL3120793 GS-6620 EC50 = 40000.0 nM 4.40
CHEMBL4065501 EC50 = 56000.0 nM 4.25
CHEMBL3120779 EC50 > 10000.0 nM -
CHEMBL4090316 EC50 > 10000.0 nM -
CHEMBL2016758 EC50 > 10000.0 nM -