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Assay Detail

CHEMBL4013233

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of equine serum BChE using butyrylthiocholine iodide as substrate preincubated for 5 mins followed by substrate addition measured every minute for 10 mins by Ellman's spectrophotometric method
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Equus caballus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL5763)
Type SINGLE PROTEIN
Organism Equus caballus

Publication

Donepezil-Based Central Acetylcholinesterase Inhibitors by Means of a "Bio-Oxidizable" Prodrug Strategy: Design, Synthesis, and in Vitro Biological Evaluation.
Peauger L, Azzouz R, Gembus V, Ţînţaş ML, Sopková-de Oliveira Santos J, Bohn P, Papamicaël C, Levacher V.
J Med Chem (2017) 60 : 5909 -5926
PubMed 28613859 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.81 8.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL95 TACRINE 4.0 1 8.49
CHEMBL4074062 1 6.58
CHEMBL4084371 1 5.79
CHEMBL4095535 1 5.75
CHEMBL4066548 1 5.72
CHEMBL4094591 1 5.52
CHEMBL4068149 1 5.51
CHEMBL4102333 1 5.48
CHEMBL4099873 1 5.44
CHEMBL4072999 1 5.42
CHEMBL4064413 1 5.32
CHEMBL4081915 1 5.23
CHEMBL4094081 1 5.22
CHEMBL4076930 1 -
CHEMBL4092048 1 -
CHEMBL4098418 1 -
CHEMBL4092123 1 -
CHEMBL4060011 1 -
CHEMBL4071377 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL95 TACRINE IC50 = 3.2 nM 8.49
CHEMBL4074062 IC50 = 262.0 nM 6.58
CHEMBL4084371 IC50 = 1615.0 nM 5.79
CHEMBL4095535 IC50 = 1792.0 nM 5.75
CHEMBL4066548 IC50 = 1915.0 nM 5.72
CHEMBL4094591 IC50 = 2990.0 nM 5.52
CHEMBL4068149 IC50 = 3055.0 nM 5.51
CHEMBL4102333 IC50 = 3340.0 nM 5.48
CHEMBL4099873 IC50 = 3630.0 nM 5.44
CHEMBL4072999 IC50 = 3815.0 nM 5.42
CHEMBL4064413 IC50 = 4765.0 nM 5.32
CHEMBL4081915 IC50 = 5930.0 nM 5.23
CHEMBL4094081 IC50 = 5990.0 nM 5.22
CHEMBL4076930 IC50 > 10000.0 nM -
CHEMBL4092048 IC50 > 10000.0 nM -
CHEMBL4098418 IC50 - -
CHEMBL4092123 IC50 - -
CHEMBL4060011 IC50 - -
CHEMBL4071377 IC50 - -