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Assay Detail

CHEMBL4013545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA assay
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

PRMT5/MEP50 complex (CHEMBL3137261)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Potent, Selective, and Cell Active Protein Arginine Methyltransferase 5 (PRMT5) Inhibitor Developed by Structure-Based Virtual Screening and Hit Optimization.
Mao R, Shao J, Zhu K, Zhang Y, Ding H, Zhang C, Shi Z, Jiang H, Sun D, Duan W, Luo C.
J Med Chem (2017) 60 : 6289 -6304
PubMed 28650658 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 5.30 6.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4092336 1 6.48
CHEMBL4078091 1 6.48
CHEMBL4071851 1 6.47
CHEMBL4060448 1 6.46
CHEMBL4102308 1 6.30
CHEMBL4096718 1 6.28
CHEMBL4093573 1 6.08
CHEMBL4065013 1 5.85
CHEMBL4103903 1 5.80
CHEMBL4098309 1 5.72
CHEMBL4097311 1 5.62
CHEMBL4069130 1 5.55
CHEMBL4074067 1 5.54
CHEMBL4063223 1 5.48
CHEMBL4063156 1 5.09
CHEMBL4096147 1 5.08
CHEMBL4071771 1 4.75
CHEMBL1289144 1 4.72
CHEMBL1412370 1 4.64
CHEMBL4094598 1 4.60
CHEMBL4060373 1 4.58
CHEMBL4070981 1 4.57
CHEMBL4082279 1 4.57
CHEMBL1290699 1 4.48
CHEMBL4085982 1 4.40
CHEMBL3590214 1 4.33
CHEMBL4068547 1 4.28
CHEMBL4077572 1 4.06
CHEMBL4092698 1 -
CHEMBL1349478 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4092336 IC50 = 330.0 nM 6.48
CHEMBL4078091 IC50 = 330.0 nM 6.48
CHEMBL4071851 IC50 = 340.0 nM 6.47
CHEMBL4060448 IC50 = 350.0 nM 6.46
CHEMBL4102308 IC50 = 500.0 nM 6.30
CHEMBL4096718 IC50 = 530.0 nM 6.28
CHEMBL4093573 IC50 = 830.0 nM 6.08
CHEMBL4065013 IC50 = 1400.0 nM 5.85
CHEMBL4103903 IC50 = 1600.0 nM 5.80
CHEMBL4098309 IC50 = 1900.0 nM 5.72
CHEMBL4097311 IC50 = 2400.0 nM 5.62
CHEMBL4069130 IC50 = 2800.0 nM 5.55
CHEMBL4074067 IC50 = 2900.0 nM 5.54
CHEMBL4063223 IC50 = 3300.0 nM 5.48
CHEMBL4063156 IC50 = 8100.0 nM 5.09
CHEMBL4096147 IC50 = 8400.0 nM 5.08
CHEMBL4071771 IC50 = 18000.0 nM 4.75
CHEMBL1289144 IC50 = 19000.0 nM 4.72
CHEMBL1412370 IC50 = 23000.0 nM 4.64
CHEMBL4094598 IC50 = 25000.0 nM 4.60
CHEMBL4060373 IC50 = 26000.0 nM 4.58
CHEMBL4070981 IC50 = 27000.0 nM 4.57
CHEMBL4082279 IC50 = 27000.0 nM 4.57
CHEMBL1290699 IC50 = 33000.0 nM 4.48
CHEMBL4085982 IC50 = 40000.0 nM 4.40
CHEMBL3590214 IC50 = 47000.0 nM 4.33
CHEMBL4068547 IC50 = 52000.0 nM 4.28
CHEMBL4077572 IC50 = 87000.0 nM 4.06
CHEMBL4084944 IC50 > 100000.0 nM -
CHEMBL1472767 IC50 > 100000.0 nM -
CHEMBL4081799 IC50 > 200000.0 nM -
CHEMBL4071261 IC50 > 200000.0 nM -
CHEMBL4102895 IC50 > 100000.0 nM -
CHEMBL4086889 IC50 > 100000.0 nM -
CHEMBL4074678 IC50 > 100000.0 nM -
CHEMBL1349478 IC50 > 200000.0 nM -
CHEMBL4092698 IC50 > 100000.0 nM -