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Assay Detail

CHEMBL4024656

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Induction of selective estrogen receptor alpha degradation in human MCF7 cells harboring TK-ERE-Luc assessed as reduction in estradiol-induced GREB1 mRNA expression after 24 hrs by TaqMan assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of LSZ102, a Potent, Orally Bioavailable Selective Estrogen Receptor Degrader (SERD) for the Treatment of Estrogen Receptor Positive Breast Cancer.
Tria GS, Abrams T, Baird J, Burks HE, Firestone B, Gaither LA, Hamann LG, He G, Kirby CA, Kim S, Lombardo F, Macchi KJ, McDonnell DP, Mishina Y, Norris JD, Nunez J, Springer C, Sun Y, Thomsen NM, Wang C, Wang J, Yu B, Tiong-Yip CL, Peukert S.
J Med Chem (2018) 61 : 2837 -2864
PubMed 29562737 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 8.15 8.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1358 FULVESTRANT 4.0 1 8.42
CHEMBL4076124 LSZ-102 1.0 1 8.05
CHEMBL489 AFIMOXIFENE 3.0 1 7.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1358 FULVESTRANT IC50 = 3.8 nM 8.42
CHEMBL4076124 LSZ-102 IC50 = 8.9 nM 8.05
CHEMBL489 AFIMOXIFENE IC50 = 10.2 nM 7.99