Assay Detail
Binding
CHEMBL4024875
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of [3H]dopamine uptake at DAT in rat brain striatal synaptosomes by liquid scintillation counting analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Tissue | Striatum |
| Subcellular | Synaptosomes |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Sodium-dependent dopamine transporter (CHEMBL338) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Covalent Modifiers: A Chemical Perspective on the Reactivity of α,β-Unsaturated Carbonyls with Thiols via Hetero-Michael Addition Reactions.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 4.31 | 4.33 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL8211 | N-ETHYLMALEIMIDE | — | 1 | 4.33 |
| CHEMBL721 | ACROLEIN | — | 1 | 4.28 |
| CHEMBL1600824 | — | — | 1 | - |
| CHEMBL9019 | ACRYLIC ACID METHYL ESTER | — | 1 | - |
| CHEMBL348107 | ACRYLAMIDE | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL8211 | N-ETHYLMALEIMIDE | IC50 | = | 46773.51 | nM | 4.33 |
| CHEMBL721 | ACROLEIN | IC50 | = | 52480.75 | nM | 4.28 |
| CHEMBL1600824 | — | IC50 | = | 331131.12 | nM | - |
| CHEMBL9019 | ACRYLIC ACID METHYL ESTER | IC50 | = | 461317574.56 | nM | - |
| CHEMBL348107 | ACRYLAMIDE | IC50 | = | 437522105.16 | nM | - |