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Assay Detail

CHEMBL4031188

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transrepression of glucocorticoid receptor in Wistar rat PBMC assessed as inhibition of LPS induced TNF alpha release preincubated for 45 mins followed by LPS addition measured after 18 hrs by ELISA
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL3368)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Selective Nonsteroidal Glucocorticoid Receptor Modulators for the Inhaled Treatment of Pulmonary Diseases.
Hemmerling M, Nilsson S, Edman K, Eirefelt S, Russell W, Hendrickx R, Johnsson E, Kärrman Mårdh C, Berger M, Rehwinkel H, Abrahamsson A, Dahmén J, Eriksson AR, Gabos B, Henriksson K, Hossain N, Ivanova S, Jansson AH, Jensen TJ, Jerre A, Johansson H, Klingstedt T, Lepistö M, Lindsjö M, Mile I, Nikitidis G, Steele J, Tehler U, Wissler L, Hansson T.
J Med Chem (2017) 60 : 8591 -8605
PubMed 28937774 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 9.64 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3666814 1 10.00
CHEMBL4072756 AZD-5423 2.0 1 9.85
CHEMBL1473 FLUTICASONE PROPIONATE 4.0 1 9.85
CHEMBL3666813 1 9.54
CHEMBL3666807 VELSECORAT 2.0 1 9.52
CHEMBL3666805 1 9.47
CHEMBL4099639 1 9.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3666814 IC50 = 0.1 nM 10.00
CHEMBL4072756 AZD-5423 IC50 = 0.14 nM 9.85
CHEMBL1473 FLUTICASONE PROPIONATE IC50 = 0.14 nM 9.85
CHEMBL3666813 IC50 = 0.29 nM 9.54
CHEMBL3666807 VELSECORAT IC50 = 0.3 nM 9.52
CHEMBL3666805 IC50 = 0.34 nM 9.47
CHEMBL4099639 IC50 = 0.56 nM 9.25