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Assay Detail

CHEMBL4040233

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-pyr-1-apelin-13 from rat C-terminal EGFP-tagged APJ receptor expressed in CHO cell membranes after 3 hrs by Wallac gamma counting
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Apelin receptor (CHEMBL2398)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthetic Modification within the "RPRL" Region of Apelin Peptides: Impact on Cardiovascular Activity and Stability to Neprilysin and Plasma Degradation.
McKinnie SMK, Wang W, Fischer C, McDonald T, Kalin KR, Iturrioz X, Llorens-Cortes C, Oudit GY, Vederas JC.
J Med Chem (2017) 60 : 6408 -6427
PubMed 28685579 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 9.66 10.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3234446 1 10.28
CHEMBL4059998 1 10.00
CHEMBL4075830 1 9.92
CHEMBL4077740 1 9.89
CHEMBL4069459 1 9.89
CHEMBL4076754 1 9.85
CHEMBL4059541 1 9.85
CHEMBL4076953 1 9.82
CHEMBL4103607 1 9.77
CHEMBL4061039 1 9.74
CHEMBL4098390 1 9.72
CHEMBL3184840 1 9.52
CHEMBL4065973 1 9.35
CHEMBL4075655 1 9.25
CHEMBL4085677 1 9.00
CHEMBL4077505 1 8.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3234446 Ki = 0.053 nM 10.28
CHEMBL4059998 Ki = 0.1 nM 10.00
CHEMBL4075830 Ki = 0.12 nM 9.92
CHEMBL4077740 Ki = 0.13 nM 9.89
CHEMBL4069459 Ki = 0.13 nM 9.89
CHEMBL4076754 Ki = 0.14 nM 9.85
CHEMBL4059541 Ki = 0.14 nM 9.85
CHEMBL4076953 Ki = 0.15 nM 9.82
CHEMBL4103607 Ki = 0.17 nM 9.77
CHEMBL4061039 Ki = 0.18 nM 9.74
CHEMBL4098390 Ki = 0.19 nM 9.72
CHEMBL3184840 Ki = 0.3 nM 9.52
CHEMBL4065973 Ki = 0.45 nM 9.35
CHEMBL4075655 Ki = 0.56 nM 9.25
CHEMBL4085677 Ki = 1.0 nM 9.00
CHEMBL4077505 Ki = 2.0 nM 8.70