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Assay Detail

CHEMBL4048639

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of full length GST-tagged recombinant human MNK1 expressed in insect cells using Ac-TATKSGSTTKNR-NH2 as substrate preincubated for 5 mins followed by ATP addition measured after 40 mins by ADP-Glo assay
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Structure-based Design of Pyridone-Aminal eFT508 Targeting Dysregulated Translation by Selective Mitogen-activated Protein Kinase Interacting Kinases 1 and 2 (MNK1/2) Inhibition.
Reich SH, Sprengeler PA, Chiang GG, Appleman JR, Chen J, Clarine J, Eam B, Ernst JT, Han Q, Goel VK, Han EZR, Huang V, Hung INJ, Jemison A, Jessen KA, Molter J, Murphy D, Neal M, Parker GS, Shaghafi M, Sperry S, Staunton J, Stumpf CR, Thompson PA, Tran C, Webber SE, Wegerski CJ, Zheng H, Webster KR.
J Med Chem (2018) 61 : 3516 -3540
PubMed 29526098 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 7.54 9.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4073743 1 9.29
CHEMBL4076439 1 9.19
CHEMBL4094975 1 9.06
CHEMBL4065802 1 8.85
CHEMBL4094080 1 8.82
CHEMBL4064839 1 8.80
CHEMBL4100554 1 8.72
CHEMBL4067557 1 8.66
CHEMBL4073443 TOMIVOSERTIB 2.0 1 8.62
CHEMBL4086319 1 8.57
CHEMBL4084102 1 8.48
CHEMBL4091862 1 8.19
CHEMBL4062387 1 8.00
CHEMBL4077341 1 7.64
CHEMBL4099497 1 7.55
CHEMBL1241463 1 7.22
CHEMBL4071021 1 7.22
CHEMBL4086042 1 7.00
CHEMBL4071844 1 6.16
CHEMBL1240591 1 5.75
CHEMBL4065855 1 5.52
CHEMBL4101302 1 5.42
CHEMBL4063216 1 5.33
CHEMBL1241462 1 5.26
CHEMBL4091092 1 5.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4073743 IC50 = 0.51 nM 9.29
CHEMBL4076439 IC50 = 0.65 nM 9.19
CHEMBL4094975 IC50 = 0.88 nM 9.06
CHEMBL4065802 IC50 = 1.4 nM 8.85
CHEMBL4094080 IC50 = 1.5 nM 8.82
CHEMBL4064839 IC50 = 1.6 nM 8.80
CHEMBL4100554 IC50 = 1.9 nM 8.72
CHEMBL4067557 IC50 = 2.2 nM 8.66
CHEMBL4073443 TOMIVOSERTIB IC50 = 2.4 nM 8.62
CHEMBL4086319 IC50 = 2.7 nM 8.57
CHEMBL4084102 IC50 = 3.3 nM 8.48
CHEMBL4091862 IC50 = 6.5 nM 8.19
CHEMBL4062387 IC50 = 10.0 nM 8.00
CHEMBL4077341 IC50 = 23.0 nM 7.64
CHEMBL4099497 IC50 = 28.0 nM 7.55
CHEMBL1241463 IC50 = 60.0 nM 7.22
CHEMBL4071021 IC50 = 60.0 nM 7.22
CHEMBL4086042 IC50 = 100.0 nM 7.00
CHEMBL4071844 IC50 = 690.0 nM 6.16
CHEMBL1240591 IC50 = 1800.0 nM 5.75
CHEMBL4065855 IC50 = 3000.0 nM 5.52
CHEMBL4101302 IC50 = 3800.0 nM 5.42
CHEMBL4063216 IC50 = 4700.0 nM 5.33
CHEMBL1241462 IC50 = 5500.0 nM 5.26
CHEMBL4091092 IC50 = 6400.0 nM 5.19