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Assay Detail

CHEMBL4048727

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vivo inhibition of MNK1/2 in human TMD8 cells xenografted in NOD SCID mouse assessed as reduction in eIF4E phosphorylation at Ser209 administered as po qd for 14 days measured over 24 hrs post last dose by Western blot method
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Confidence 1 — Organism
Curated By Autocuration

Target

Mus musculus (CHEMBL375)
Type ORGANISM
Organism Mus musculus

Publication

Structure-based Design of Pyridone-Aminal eFT508 Targeting Dysregulated Translation by Selective Mitogen-activated Protein Kinase Interacting Kinases 1 and 2 (MNK1/2) Inhibition.
Reich SH, Sprengeler PA, Chiang GG, Appleman JR, Chen J, Clarine J, Eam B, Ernst JT, Han Q, Goel VK, Han EZR, Huang V, Hung INJ, Jemison A, Jessen KA, Molter J, Murphy D, Neal M, Parker GS, Shaghafi M, Sperry S, Staunton J, Stumpf CR, Thompson PA, Tran C, Webber SE, Wegerski CJ, Zheng H, Webster KR.
J Med Chem (2018) 61 : 3516 -3540
PubMed 29526098 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.80 7.80
IC90 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4073443 TOMIVOSERTIB 2.0 2 7.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4073443 TOMIVOSERTIB IC50 = 15.8 nM 7.80
CHEMBL4073443 TOMIVOSERTIB IC90 = 376.0 nM -