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Assay Detail

CHEMBL4048893

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NMS from human muscarinic M5 receptor expressed in CHO cell membranes after 2 hrs by liquid scintillation counting method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M5 (CHEMBL2035)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1-[3-(4-Butylpiperidin-1-yl)propyl]-1,2,3,4-tetrahydroquinolin-2-one (77-LH-28-1) as a Model for the Rational Design of a Novel Class of Brain Penetrant Ligands with High Affinity and Selectivity for Dopamine D4 Receptor.
Del Bello F, Bonifazi A, Giorgioni G, Cifani C, Micioni Di Bonaventura MV, Petrelli R, Piergentili A, Fontana S, Mammoli V, Yano H, Matucci R, Vistoli G, Quaglia W.
J Med Chem (2018) 61 : 3712 -3725
PubMed 29589445 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 5.29 6.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4093527 1 6.12
CHEMBL4098720 1 5.71
CHEMBL4091231 1 5.69
CHEMBL4080675 1 5.45
CHEMBL4072818 1 5.43
CHEMBL4091058 1 5.22
CHEMBL4063176 1 5.10
CHEMBL4064222 1 5.04
CHEMBL4074104 1 4.62
CHEMBL4085780 1 4.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4093527 Ki = 758.58 nM 6.12
CHEMBL4098720 Ki = 1949.84 nM 5.71
CHEMBL4091231 Ki = 2041.74 nM 5.69
CHEMBL4080675 Ki = 3548.13 nM 5.45
CHEMBL4072818 Ki = 3715.35 nM 5.43
CHEMBL4091058 Ki = 6025.6 nM 5.22
CHEMBL4063176 Ki = 7943.28 nM 5.10
CHEMBL4064222 Ki = 9120.11 nM 5.04
CHEMBL4074104 Ki = 23988.33 nM 4.62
CHEMBL4085780 Ki = 27542.29 nM 4.56