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Assay Detail

CHEMBL4132766

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal GST tagged human PDGFRbeta (557 to 1106 end residues) cytoplasmic domain expressed in baculovirus expression system preincubated for 10 mins followed by fluorescein amidite-labelled peptide substrate addition measured after 1 hr by mobility shift assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Platelet-derived growth factor receptor beta (CHEMBL1913)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel methyl indolinone-6-carboxylates containing an indole moiety as angiokinase inhibitors.
Qin M, Tian Y, Sun X, Yu S, Xia J, Gong P, Zhang H, Zhao Y.
Eur J Med Chem (2017) 139 : 492 -502
PubMed 28826084 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.27 8.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502835 NINTEDANIB 4.0 1 8.46
CHEMBL4165429 1 7.66
CHEMBL4173078 1 7.46
CHEMBL4161991 1 7.41
CHEMBL4166696 1 7.36
CHEMBL4169257 1 7.34
CHEMBL4162400 1 7.31
CHEMBL4164318 1 7.22
CHEMBL4177112 1 7.21
CHEMBL4172205 1 7.19
CHEMBL4165850 1 7.04
CHEMBL4173756 1 7.03
CHEMBL4173817 1 6.93
CHEMBL4176458 1 6.87
CHEMBL4161351 1 6.62
CHEMBL4165827 1 -
CHEMBL4176039 1 -
CHEMBL4163106 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502835 NINTEDANIB IC50 = 3.5 nM 8.46
CHEMBL4165429 IC50 = 22.0 nM 7.66
CHEMBL4173078 IC50 = 34.3 nM 7.46
CHEMBL4161991 IC50 = 39.0 nM 7.41
CHEMBL4166696 IC50 = 44.2 nM 7.36
CHEMBL4169257 IC50 = 45.5 nM 7.34
CHEMBL4162400 IC50 = 49.2 nM 7.31
CHEMBL4164318 IC50 = 60.7 nM 7.22
CHEMBL4177112 IC50 = 61.0 nM 7.21
CHEMBL4172205 IC50 = 64.6 nM 7.19
CHEMBL4165850 IC50 = 91.7 nM 7.04
CHEMBL4173756 IC50 = 92.7 nM 7.03
CHEMBL4173817 IC50 = 116.2 nM 6.93
CHEMBL4176458 IC50 = 135.1 nM 6.87
CHEMBL4161351 IC50 = 241.6 nM 6.62
CHEMBL4165827 IC50 - -
CHEMBL4176039 IC50 - -
CHEMBL4163106 IC50 > 500.0 nM -