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Assay Detail

CHEMBL4143516

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to Clostridium difficile [5'-32P]guaA riboswitch by polyacrylamide gel electrophoresis method
42
Total Activities
42
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Clostridioides difficile
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Purine analogs targeting the guanine riboswitch as potential antibiotics against Clostridioides difficile.
Yan LH, Le Roux A, Boyapelly K, Lamontagne AM, Archambault MA, Picard-Jean F, Lalonde-Seguin D, St-Pierre E, Najmanovich RJ, Fortier LC, Lafontaine D, Marsault É.
Eur J Med Chem (2018) 143 : 755 -768
PubMed 29220796 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 42 5.32 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL219568 GUANINE 3.0 1 8.70
CHEMBL4164786 1 6.50
CHEMBL4161199 1 6.35
CHEMBL4171810 1 6.25
CHEMBL4161375 1 6.20
CHEMBL133214 1 6.03
CHEMBL1224659 1 5.98
CHEMBL4168054 1 5.74
CHEMBL4176476 1 5.69
CHEMBL4176187 1 5.66
CHEMBL4168998 1 5.62
CHEMBL4169532 1 5.57
CHEMBL305113 1 5.40
CHEMBL4161073 1 5.19
CHEMBL4159196 1 5.13
CHEMBL1230674 9-Methylguanine 1 5.09
CHEMBL4165572 1 5.01
CHEMBL4176070 1 4.91
CHEMBL4160133 1 4.79
CHEMBL4163504 1 4.68
CHEMBL4163624 1 4.61
CHEMBL4170763 1 4.60
CHEMBL1867153 1 4.60
CHEMBL4165864 1 4.56
CHEMBL4161615 1 4.53
CHEMBL4172681 1 4.32
CHEMBL4168221 1 4.17
CHEMBL4162822 1 4.16
CHEMBL1864419 1 4.15
CHEMBL4169051 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL219568 GUANINE Kd = 2.0 nM 8.70
CHEMBL4164786 Kd = 320.0 nM 6.50
CHEMBL4161199 Kd = 450.0 nM 6.35
CHEMBL4171810 Kd = 560.0 nM 6.25
CHEMBL4161375 Kd = 630.0 nM 6.20
CHEMBL133214 Kd = 930.0 nM 6.03
CHEMBL1224659 Kd = 1050.0 nM 5.98
CHEMBL4168054 Kd = 1810.0 nM 5.74
CHEMBL4176476 Kd = 2060.0 nM 5.69
CHEMBL4176187 Kd = 2200.0 nM 5.66
CHEMBL4168998 Kd = 2400.0 nM 5.62
CHEMBL4169532 Kd = 2670.0 nM 5.57
CHEMBL305113 Kd = 4000.0 nM 5.40
CHEMBL4161073 Kd = 6500.0 nM 5.19
CHEMBL4159196 Kd = 7400.0 nM 5.13
CHEMBL1230674 9-Methylguanine Kd = 8100.0 nM 5.09
CHEMBL4165572 Kd = 9800.0 nM 5.01
CHEMBL4176070 Kd = 12300.0 nM 4.91
CHEMBL4160133 Kd = 16400.0 nM 4.79
CHEMBL4163504 Kd = 21000.0 nM 4.68
CHEMBL4163624 Kd = 24400.0 nM 4.61
CHEMBL4170763 Kd = 25000.0 nM 4.60
CHEMBL1867153 Kd = 25200.0 nM 4.60
CHEMBL4165864 Kd = 27600.0 nM 4.56
CHEMBL4161615 Kd = 29400.0 nM 4.53
CHEMBL4172681 Kd = 48300.0 nM 4.32
CHEMBL4168221 Kd = 68300.0 nM 4.17
CHEMBL4162822 Kd = 68900.0 nM 4.16
CHEMBL1864419 Kd = 71300.0 nM 4.15
CHEMBL4175253 Kd > 1000000.0 nM -
CHEMBL4174303 Kd = 300300.0 nM -
CHEMBL4175830 Kd = 139900.0 nM -
CHEMBL4162135 Kd > 1000000.0 nM -
CHEMBL33416 Kd > 1000000.0 nM -
CHEMBL4175789 Kd > 1000000.0 nM -
CHEMBL4167932 Kd > 1000000.0 nM -
CHEMBL4169294 Kd > 1000000.0 nM -
CHEMBL4173274 Kd > 1000000.0 nM -
CHEMBL4162581 Kd > 1000000.0 nM -
CHEMBL4165218 Kd > 1000000.0 nM -
CHEMBL4171397 Kd > 1000000.0 nM -
CHEMBL4169051 Kd > 1000000.0 nM -