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Assay Detail

CHEMBL4151113

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human sPLA2-10 expressed in Escherichia coli BL21(DE3) using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine as substrate pretreated for 20 mins followed by substrate addition and measured after 60 mins
32
Total Activities
32
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Group 10 secretory phospholipase A2 (CHEMBL4342)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a Series of Indole-2 Carboxamides as Selective Secreted Phospholipase A2 Type X (sPLA2-X) Inhibitors.
Knerr L, Giordanetto F, Nordberg P, Pettersen D, Selmi N, Beisel HG, de la Motte H, Olsson T, Perkins TDJ, Herslöf M, Månsson Å, Dahlström M, Starke I, Broddefalk J, Saarinen G, Klingegård F, Hurt-Camejo E, Rosengren B, Brengdahl J, Jansen F, Rohman M, Sandmark J, Hallberg K, Åkerud T, Roth RG, Ahlqvist M.
ACS Med Chem Lett (2018) 9 : 594 -599
PubMed 30034585 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 27 6.06 7.58
Inhibition 5 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4171084 1 7.58
CHEMBL148674 VARESPLADIB 2.0 1 7.39
CHEMBL4176544 1 7.37
CHEMBL4160483 1 7.17
CHEMBL4172222 1 7.05
CHEMBL4175583 1 7.03
CHEMBL4174522 1 6.96
CHEMBL4173359 1 6.80
CHEMBL4175643 1 6.77
CHEMBL4171797 1 6.64
CHEMBL4161605 1 6.58
CHEMBL4160502 1 6.21
CHEMBL4159939 1 6.08
CHEMBL4176269 1 6.00
CHEMBL4170085 1 5.72
CHEMBL4168202 1 5.57
CHEMBL4163859 1 5.55
CHEMBL4172025 1 5.32
CHEMBL4169491 1 5.16
CHEMBL4168275 1 5.14
CHEMBL4176688 1 4.99
CHEMBL4175476 1 4.68
CHEMBL4168405 1 4.63
CHEMBL4160366 1 4.57
CHEMBL4166071 1 4.54
CHEMBL4164836 1 -
CHEMBL4161571 1 -
CHEMBL4176533 1 -
CHEMBL4161426 1 -
CHEMBL4168676 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4171084 IC50 = 26.0 nM 7.58
CHEMBL148674 VARESPLADIB IC50 = 41.0 nM 7.39
CHEMBL4176544 IC50 = 43.0 nM 7.37
CHEMBL4160483 IC50 = 68.0 nM 7.17
CHEMBL4172222 IC50 = 90.0 nM 7.05
CHEMBL4175583 IC50 = 93.0 nM 7.03
CHEMBL4174522 IC50 = 110.0 nM 6.96
CHEMBL4173359 IC50 = 160.0 nM 6.80
CHEMBL4175643 IC50 = 170.0 nM 6.77
CHEMBL4171797 IC50 = 230.0 nM 6.64
CHEMBL4161605 IC50 = 260.0 nM 6.58
CHEMBL4160502 IC50 = 620.0 nM 6.21
CHEMBL4159939 IC50 = 830.0 nM 6.08
CHEMBL4176269 IC50 = 990.0 nM 6.00
CHEMBL4170085 IC50 = 1900.0 nM 5.72
CHEMBL4168202 IC50 = 2700.0 nM 5.57
CHEMBL4163859 IC50 = 2800.0 nM 5.55
CHEMBL4172025 IC50 = 4800.0 nM 5.32
CHEMBL4169491 IC50 = 6900.0 nM 5.16
CHEMBL4168275 IC50 = 7200.0 nM 5.14
CHEMBL4176688 IC50 = 10300.0 nM 4.99
CHEMBL4175476 IC50 = 20700.0 nM 4.68
CHEMBL4168405 IC50 = 23600.0 nM 4.63
CHEMBL4160366 IC50 = 26900.0 nM 4.57
CHEMBL4166071 IC50 = 29100.0 nM 4.54
CHEMBL4164836 IC50 > 30000.0 nM -
CHEMBL4161571 IC50 > 30000.0 nM -
CHEMBL4176533 Inhibition - % -
CHEMBL4161426 Inhibition - % -
CHEMBL4168676 Inhibition - % -
CHEMBL4173162 Inhibition - % -
CHEMBL4165255 Inhibition - % -