Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4178244

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human full length BTK preincubated for 10 mins followed by Tk-biotin peptide substrate addition after 90 mins by TR-FRET assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and evaluation of 1H-pyrrolo[2,3-b]pyridine based selective and reversible small molecule BTK inhibitors for the treatment of rheumatoid arthritis.
Thakkar M, Bhuniya D, Kaduskar R, Mengawade T, Naik K, Salunkhe V, Bhalerao A, Kurhade S, Mavinahalli J, Jain V, Petla R, Avaragolla S, Ray S, Rouduri S, Dhanave A, De S, Pathade V, Tambe A, Raje AA, Madgula V, Joshi S, Nadeem A, Bala M, Umrani D, Hariharan N, Kulkarni B, Mookhtiar KA.
Bioorg Med Chem Lett (2017) 27 : 1867 -1873
PubMed 28279528 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 8.46 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3928203 1 9.00
CHEMBL3936823 1 9.00
CHEMBL3982806 1 8.96
CHEMBL4216939 1 8.92
CHEMBL3946460 1 8.85
CHEMBL3959033 1 8.77
CHEMBL3967108 1 8.59
CHEMBL3900184 1 8.59
CHEMBL3966346 1 8.51
CHEMBL3962146 1 8.29
CHEMBL3922230 1 8.27
CHEMBL3891434 1 8.26
CHEMBL3891185 1 8.22
CHEMBL3921350 1 8.19
CHEMBL3907763 1 8.17
CHEMBL3935716 1 8.08
CHEMBL3968892 1 7.93
CHEMBL3968928 1 7.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3928203 IC50 = 1.0 nM 9.00
CHEMBL3936823 IC50 = 1.0 nM 9.00
CHEMBL3982806 IC50 = 1.1 nM 8.96
CHEMBL4216939 IC50 = 1.2 nM 8.92
CHEMBL3946460 IC50 = 1.4 nM 8.85
CHEMBL3959033 IC50 = 1.7 nM 8.77
CHEMBL3967108 IC50 = 2.6 nM 8.59
CHEMBL3900184 IC50 = 2.6 nM 8.59
CHEMBL3966346 IC50 = 3.1 nM 8.51
CHEMBL3962146 IC50 = 5.1 nM 8.29
CHEMBL3922230 IC50 = 5.4 nM 8.27
CHEMBL3891434 IC50 = 5.5 nM 8.26
CHEMBL3891185 IC50 = 6.0 nM 8.22
CHEMBL3921350 IC50 = 6.5 nM 8.19
CHEMBL3907763 IC50 = 6.8 nM 8.17
CHEMBL3935716 IC50 = 8.3 nM 8.08
CHEMBL3968892 IC50 = 11.7 nM 7.93
CHEMBL3968928 IC50 = 21.0 nM 7.68