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Assay Detail

CHEMBL4198706

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS coronavirus 3CL protease using Dabcyl-KTSAVLQ/SGFRKME-Edans as substrate incubated for 30 mins followed by substrate addition measured after 1 hr by FRET assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome-related coronavirus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Replicase polyprotein 1ab (CHEMBL5118)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome-related coronavirus

Publication

Structure-guided design of potent and permeable inhibitors of MERS coronavirus 3CL protease that utilize a piperidine moiety as a novel design element.
Galasiti Kankanamalage AC, Kim Y, Damalanka VC, Rathnayake AD, Fehr AR, Mehzabeen N, Battaile KP, Lovell S, Lushington GH, Perlman S, Chang KO, Groutas WC.
Eur J Med Chem (2018) 150 : 334 -346
PubMed 29544147 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.20 5.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4214066 1 5.68
CHEMBL4217568 1 5.50
CHEMBL4214471 1 5.41
CHEMBL4216101 1 5.39
CHEMBL4208764 1 5.37
CHEMBL4208240 1 5.29
CHEMBL4202812 1 5.28
CHEMBL4212620 1 5.26
CHEMBL4203883 1 5.20
CHEMBL4204431 1 5.06
CHEMBL4209146 1 4.54
CHEMBL4213159 1 4.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4214066 IC50 = 2100.0 nM 5.68
CHEMBL4217568 IC50 = 3200.0 nM 5.50
CHEMBL4214471 IC50 = 3900.0 nM 5.41
CHEMBL4216101 IC50 = 4100.0 nM 5.39
CHEMBL4208764 IC50 = 4300.0 nM 5.37
CHEMBL4208240 IC50 = 5100.0 nM 5.29
CHEMBL4202812 IC50 = 5200.0 nM 5.28
CHEMBL4212620 IC50 = 5500.0 nM 5.26
CHEMBL4203883 IC50 = 6300.0 nM 5.20
CHEMBL4204431 IC50 = 8800.0 nM 5.06
CHEMBL4209146 IC50 = 28800.0 nM 4.54
CHEMBL4213159 IC50 = 42100.0 nM 4.38