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Assay Detail

CHEMBL4220744

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of equine serum BuChE using S-butyrylthiocholine chloride as substrate preincubated for 15 mins followed by substrate addition and measured for 45 mins by Ellmans microplate assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Equus caballus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cholinesterase (CHEMBL5763)
Type SINGLE PROTEIN
Organism Equus caballus

Publication

In silico studies, synthesis and pharmacological evaluation to explore multi-targeted approach for imidazole analogues as potential cholinesterase inhibitors with neuroprotective role for Alzheimer's disease.
Gurjar AS, Darekar MN, Yeong KY, Ooi L.
Bioorg Med Chem (2018) 26 : 1511 -1522
PubMed 29429576 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.39 5.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL659 GALANTAMINE 4.0 1 5.48
CHEMBL601724 1 5.30
CHEMBL4227873 1 -
CHEMBL502 DONEPEZIL 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL659 GALANTAMINE IC50 = 3310.0 nM 5.48
CHEMBL601724 IC50 = 4990.0 nM 5.30
CHEMBL4227873 IC50 - -
CHEMBL502 DONEPEZIL IC50 - -