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Assay Detail

CHEMBL4233071

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of chymotrypsin-like activity of human 20S proteasome using Suc-Leu-Leu-Val-Tyr-AMC as substrate incubated for 10 mins followed by substrate addition measured after 1 hr by spectrofluorimetric method
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome Macropain subunit MB1 (CHEMBL4662)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, in vitro and in vivo evaluation, and structure-activity relationship (SAR) discussion of novel dipeptidyl boronic acid proteasome inhibitors as orally available anti-cancer agents for the treatment of multiple myeloma and mechanism studies.
Lei M, Feng H, Bai E, Zhou H, Wang J, Shi J, Wang X, Hu S, Liu Z, Zhu Y.
Bioorg Med Chem (2018) 26 : 3975 -3981
PubMed 29934218 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.84 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4249022 1 8.70
CHEMBL4246480 1 8.64
CHEMBL4238198 1 8.49
CHEMBL4246864 1 8.45
CHEMBL4241473 1 8.38
CHEMBL4251272 1 8.32
CHEMBL4245244 1 8.28
CHEMBL4238378 1 8.23
CHEMBL4248209 1 8.17
CHEMBL325041 BORTEZOMIB 3.0 1 8.15
CHEMBL2141296 IXAZOMIB 3.0 1 8.11
CHEMBL4243375 1 8.09
CHEMBL4242666 1 8.09
CHEMBL1813256 1 8.07
CHEMBL4249503 1 8.05
CHEMBL4249917 1 7.89
CHEMBL4238931 1 7.69
CHEMBL4248689 1 7.67
CHEMBL4246922 1 7.57
CHEMBL4243132 1 7.56
CHEMBL4249453 1 7.51
CHEMBL4239365 1 7.37
CHEMBL4250217 1 7.13
CHEMBL1813227 1 6.52
CHEMBL4244424 1 6.41
CHEMBL4245648 1 6.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4249022 IC50 = 1.98 nM 8.70
CHEMBL4246480 IC50 = 2.31 nM 8.64
CHEMBL4238198 IC50 = 3.22 nM 8.49
CHEMBL4246864 IC50 = 3.53 nM 8.45
CHEMBL4241473 IC50 = 4.16 nM 8.38
CHEMBL4251272 IC50 = 4.82 nM 8.32
CHEMBL4245244 IC50 = 5.26 nM 8.28
CHEMBL4238378 IC50 = 5.89 nM 8.23
CHEMBL4248209 IC50 = 6.74 nM 8.17
CHEMBL325041 BORTEZOMIB IC50 = 7.04 nM 8.15
CHEMBL2141296 IXAZOMIB IC50 = 7.73 nM 8.11
CHEMBL4243375 IC50 = 8.21 nM 8.09
CHEMBL4242666 IC50 = 8.11 nM 8.09
CHEMBL1813256 IC50 = 8.44 nM 8.07
CHEMBL4249503 IC50 = 8.86 nM 8.05
CHEMBL4249917 IC50 = 12.89 nM 7.89
CHEMBL4238931 IC50 = 20.44 nM 7.69
CHEMBL4248689 IC50 = 21.17 nM 7.67
CHEMBL4246922 IC50 = 26.79 nM 7.57
CHEMBL4243132 IC50 = 27.75 nM 7.56
CHEMBL4249453 IC50 = 30.78 nM 7.51
CHEMBL4239365 IC50 = 42.79 nM 7.37
CHEMBL4250217 IC50 = 74.86 nM 7.13
CHEMBL1813227 IC50 = 303.8 nM 6.52
CHEMBL4244424 IC50 = 387.0 nM 6.41
CHEMBL4245648 IC50 = 593.7 nM 6.23