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Assay Detail

CHEMBL4236761

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MPS1 autophosphorylation at Thr33/Ser37 in human HCT116 cells after 2 hrs by MSD assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity protein kinase TTK (CHEMBL3983)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Introduction of a Methyl Group Curbs Metabolism of Pyrido[3,4- d]pyrimidine Monopolar Spindle 1 (MPS1) Inhibitors and Enables the Discovery of the Phase 1 Clinical Candidate N2-(2-Ethoxy-4-(4-methyl-4 H-1,2,4-triazol-3-yl)phenyl)-6-methyl- N8-neopentylpyrido[3,4- d]pyrimidine-2,8-diamine (BOS172722).
Woodward HL, Innocenti P, Cheung KJ, Hayes A, Roberts J, Henley AT, Faisal A, Mak GW, Box G, Westwood IM, Cronin N, Carter M, Valenti M, De Haven Brandon A, O'Fee L, Saville H, Schmitt J, Burke R, Broccatelli F, van Montfort RLM, Raynaud FI, Eccles SA, Linardopoulos S, Blagg J, Hoelder S.
J Med Chem (2018) 61 : 8226 -8240
PubMed 30199249 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.03 8.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3890427 1 8.46
CHEMBL4249882 1 7.64
CHEMBL3932996 1 7.52
CHEMBL4247121 1 7.48
CHEMBL4245639 1 7.43
CHEMBL4239476 1 7.43
CHEMBL3911532 1 7.36
CHEMBL3924132 BOS-172722 1.0 1 7.20
CHEMBL3900491 1 7.02
CHEMBL4249151 1 6.96
CHEMBL3809252 1 6.85
CHEMBL3927553 1 6.77
CHEMBL3961036 1 6.64
CHEMBL4240502 1 6.64
CHEMBL4251352 1 6.62
CHEMBL4250961 1 6.60
CHEMBL4248872 1 6.43
CHEMBL4242054 1 5.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3890427 IC50 = 3.5 nM 8.46
CHEMBL4249882 IC50 = 23.0 nM 7.64
CHEMBL3932996 IC50 = 30.0 nM 7.52
CHEMBL4247121 IC50 = 33.0 nM 7.48
CHEMBL4245639 IC50 = 37.0 nM 7.43
CHEMBL4239476 IC50 = 37.0 nM 7.43
CHEMBL3911532 IC50 = 44.0 nM 7.36
CHEMBL3924132 BOS-172722 IC50 = 63.0 nM 7.20
CHEMBL3900491 IC50 = 95.0 nM 7.02
CHEMBL4249151 IC50 = 110.0 nM 6.96
CHEMBL3809252 IC50 = 140.0 nM 6.85
CHEMBL3927553 IC50 = 170.0 nM 6.77
CHEMBL3961036 IC50 = 230.0 nM 6.64
CHEMBL4240502 IC50 = 230.0 nM 6.64
CHEMBL4251352 IC50 = 240.0 nM 6.62
CHEMBL4250961 IC50 = 250.0 nM 6.60
CHEMBL4248872 IC50 = 370.0 nM 6.43
CHEMBL4242054 IC50 = 3800.0 nM 5.42