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Assay Detail

CHEMBL4265665

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive displacement of fluorescently labelled PPAR tracer ligand from GST-tagged human PPARgamma ligand binding domain after 1 hr in dark by TR-FRET competitive binding assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

PPARγ-sparing thiazolidinediones as insulin sensitizers. Design, synthesis and selection of compounds for clinical development.
Tanis SP, Colca JR, Parker TT, Artman GD, Larsen SD, McDonald WG, Gadwood RC, Kletzien RF, Zeller JB, Lee PH, Adams WJ.
Bioorg Med Chem (2018) 26 : 5870 -5884
PubMed 30429097 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 5.42 6.95

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL121 ROSIGLITAZONE 4.0 1 6.95
CHEMBL186179 MURAGLITAZAR 3.0 1 6.92
CHEMBL408 TROGLITAZONE 4.0 1 6.43
CHEMBL595 PIOGLITAZONE 4.0 1 5.75
CHEMBL4276678 1 5.63
CHEMBL4293295 1 5.51
CHEMBL4288064 1 5.07
CHEMBL4280830 1 4.93
CHEMBL4291468 1 4.74
CHEMBL146624 MITOGLITAZONE 2.0 1 4.65
CHEMBL4284250 1 4.30
CHEMBL4286980 1 4.11
CHEMBL7002 CIGLITAZONE 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL121 ROSIGLITAZONE IC50 = 112.0 nM 6.95
CHEMBL186179 MURAGLITAZAR IC50 = 120.0 nM 6.92
CHEMBL408 TROGLITAZONE IC50 = 370.0 nM 6.43
CHEMBL595 PIOGLITAZONE IC50 = 1790.0 nM 5.75
CHEMBL4276678 IC50 = 2320.0 nM 5.63
CHEMBL4293295 IC50 = 3120.0 nM 5.51
CHEMBL4288064 IC50 = 8460.0 nM 5.07
CHEMBL4280830 IC50 = 11700.0 nM 4.93
CHEMBL4291468 IC50 = 18140.0 nM 4.74
CHEMBL146624 MITOGLITAZONE IC50 = 22300.0 nM 4.65
CHEMBL4284250 IC50 = 50140.0 nM 4.30
CHEMBL4286980 IC50 = 78450.0 nM 4.11
CHEMBL7002 CIGLITAZONE IC50 > 5000.0 nM -