Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4273104

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human TNAP expressed in COS7 cells using CDP-star as substrate preincubated for 5 to 10 mins followed by substrate addition measured after 20 mins by luminescence-based assay
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alkaline phosphatase, tissue-nonspecific isozyme (CHEMBL5979)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Exploration of carboxy pyrazole derivatives: Synthesis, alkaline phosphatase, nucleotide pyrophosphatase/phosphodiesterase and nucleoside triphosphate diphosphohydrolase inhibition studies with potential anticancer profile.
Channar PA, Afzal S, Ejaz SA, Saeed A, Larik FA, Mahesar PA, Lecka J, Sévigny J, Erben MF, Iqbal J.
Eur J Med Chem (2018) 156 : 461 -478
PubMed 30015078 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 5.18 6.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL243638 1 6.04
CHEMBL4293491 1 5.97
CHEMBL4279910 1 5.82
CHEMBL4295005 1 5.59
CHEMBL4283075 1 5.45
CHEMBL4286443 1 5.34
CHEMBL4283786 1 5.31
CHEMBL4280333 1 5.26
CHEMBL4288229 1 5.24
CHEMBL390695 1 5.24
CHEMBL4284994 1 5.21
CHEMBL298036 1 5.19
CHEMBL4287085 1 5.11
CHEMBL4290501 1 5.08
CHEMBL4291645 1 4.95
CHEMBL4291589 1 4.88
CHEMBL4280275 1 4.75
CHEMBL4288181 1 4.72
CHEMBL1454 LEVAMISOLE 4.0 1 4.69
CHEMBL4285649 1 4.58
CHEMBL4283728 1 4.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL243638 IC50 = 910.0 nM 6.04
CHEMBL4293491 IC50 = 1070.0 nM 5.97
CHEMBL4279910 IC50 = 1510.0 nM 5.82
CHEMBL4295005 IC50 = 2590.0 nM 5.59
CHEMBL4283075 IC50 = 3580.0 nM 5.45
CHEMBL4286443 IC50 = 4580.0 nM 5.34
CHEMBL4283786 IC50 = 4910.0 nM 5.31
CHEMBL4280333 IC50 = 5430.0 nM 5.26
CHEMBL4288229 IC50 = 5780.0 nM 5.24
CHEMBL390695 IC50 = 5710.0 nM 5.24
CHEMBL4284994 IC50 = 6140.0 nM 5.21
CHEMBL298036 IC50 = 6510.0 nM 5.19
CHEMBL4287085 IC50 = 7860.0 nM 5.11
CHEMBL4290501 IC50 = 8240.0 nM 5.08
CHEMBL4291645 IC50 = 11120.0 nM 4.95
CHEMBL4291589 IC50 = 13300.0 nM 4.88
CHEMBL4280275 IC50 = 17700.0 nM 4.75
CHEMBL4288181 IC50 = 19100.0 nM 4.72
CHEMBL1454 LEVAMISOLE IC50 = 20210.0 nM 4.69
CHEMBL4285649 IC50 = 26400.0 nM 4.58
CHEMBL4283728 IC50 = 42000.0 nM 4.38