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Assay Detail

CHEMBL4308247

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Functional
Agonist activity at Gi-coupled mu opioid receptor (unknown origin) expressed in HEK293T cells assessed as inhibition of cAMP production at pH 7.4 measured after 15 mins by Glo-Sensor assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

β-Fluorofentanyls Are pH-Sensitive Mu Opioid Receptor Agonists.
Rosas R, Huang XP, Roth BL, Dockendorff C.
ACS Med Chem Lett (2019) 10 : 1353 -1356
PubMed 31531209 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.34 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL38874 DAMGO 1 9.10
CHEMBL4468844 1 8.23
CHEMBL4471584 1 8.12
CHEMBL596 FENTANYL 4.0 1 7.97
CHEMBL4458454 1 7.11
CHEMBL4550454 1 7.05
CHEMBL4516683 1 6.99
CHEMBL4441320 1 6.98
CHEMBL4537210 1 4.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL38874 DAMGO IC50 = 0.7943 nM 9.10
CHEMBL4468844 IC50 = 5.888 nM 8.23
CHEMBL4471584 IC50 = 7.586 nM 8.12
CHEMBL596 FENTANYL IC50 = 10.72 nM 7.97
CHEMBL4458454 IC50 = 77.62 nM 7.11
CHEMBL4550454 IC50 = 89.13 nM 7.05
CHEMBL4516683 IC50 = 102.33 nM 6.99
CHEMBL4441320 IC50 = 104.71 nM 6.98
CHEMBL4537210 IC50 = 31622.78 nM 4.50