Assay Detail
Binding
CHEMBL4325679
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of CYP3A5 in doxycycline-induced CYP3A5 overexpressing wild type human AsPC1 cells assessed as decrease in 1-hydroxymidazolam formation using midazolam as substrate pretreated with doxycycline for 24 hrs followed by incubation with compound for 24 hrs by LC-MS/MS analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | ASPC1 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Clobetasol Propionate Is a Heme-Mediated Selective Inhibitor of Human Cytochrome P450 3A5.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 7.27 | 7.68 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1159650 | CLOBETASOL PROPIONATE | 4.0 | 1 | 7.68 |
| CHEMBL157101 | KETOCONAZOLE | 4.0 | 1 | 6.85 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1159650 | CLOBETASOL PROPIONATE | IC50 | = | 21.0 | nM | 7.68 |
| CHEMBL157101 | KETOCONAZOLE | IC50 | = | 141.0 | nM | 6.85 |