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Assay Detail

CHEMBL4346904

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP3A4 in human liver microsomes assessed as reduction in metabolite formation using midazolam as substrate incubated for 10 mins in presence of NADPH by HPLC based mass spectrometric method
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis and structure-activity relationships of novel macrolones: Hybrids of 2-fluoro 9-oxime ketolides and carbamoyl quinolones with highly improved activity against resistant pathogens.
Ma CX, Lv W, Li YX, Fan BZ, Han X, Kong FS, Tian JC, Cushman M, Liang JH.
Eur J Med Chem (2019) 169 : 1 -20
PubMed 30852383 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.48 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL157101 KETOCONAZOLE 4.0 1 7.22
CHEMBL4459487 1 5.29
CHEMBL4537107 1 5.13
CHEMBL1136 TELITHROMYCIN 4.0 1 4.93
CHEMBL4466824 1 4.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL157101 KETOCONAZOLE IC50 = 60.0 nM 7.22
CHEMBL4459487 IC50 = 5140.0 nM 5.29
CHEMBL4537107 IC50 = 7320.0 nM 5.13
CHEMBL1136 TELITHROMYCIN IC50 = 11800.0 nM 4.93
CHEMBL4466824 IC50 = 14900.0 nM 4.83