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Assay Detail

CHEMBL4357306

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to Escherichia coli 70S ribosome assessed as reduction in BODIPY-Erythromycin A binding incubated for 1 hr by fluorescence polarization assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Subcellular Ribosome
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Bacterial 70S ribosome (CHEMBL2363135)
Type PROTEIN NUCLEIC-ACID COMPLEX
Organism Bacteria

Publication

Kinetic Target-Guided Synthesis: Reaching the Age of Maturity.
Bosc D, Camberlein V, Gealageas R, Castillo-Aguilera O, Deprez B, Deprez-Poulain R.
J Med Chem (2020) 63 : 3817 -3833
PubMed 31820982 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 3 8.92 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1232510 SOLITHROMYCIN 3.0 1 9.22
CHEMBL4463502 1 8.85
CHEMBL4203781 1 8.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1232510 SOLITHROMYCIN Kd = 0.6 nM 9.22
CHEMBL4463502 Kd = 1.4 nM 8.85
CHEMBL4203781 Kd = 2.1 nM 8.68