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Assay Detail

CHEMBL4368760

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ADMET
Binding affinity to 5'-fluorescein-tagged human mitochondrial ribosome A site mutant duplex RNA incubated for 2 mins by fluorescence spectrophotometry analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Use of a fluorescence assay to determine relative affinities of semisynthetic aminoglycosides to small RNAs representing bacterial and mitochondrial A sites.
Waduge P, Sati GC, Crich D, Chow CS.
Bioorg Med Chem (2019) 27 : 115121 -115121
PubMed 31610941 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 4 5.72 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL184618 FRAMYCETIN 3.0 1 7.00
CHEMBL370143 PAROMOMYCIN 4.0 1 5.55
CHEMBL4538701 1 5.24
CHEMBL4448326 1 5.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL184618 FRAMYCETIN Kd = 100.0 nM 7.00
CHEMBL370143 PAROMOMYCIN Kd = 2800.0 nM 5.55
CHEMBL4538701 Kd = 5800.0 nM 5.24
CHEMBL4448326 Kd = 8600.0 nM 5.07