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Assay Detail

CHEMBL4376067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Cys5-LDEETGEFL-NH2 binding to recombinant human KEAP1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3) measured after 10 to 15 mins by fluorescence polarization assay
26
Total Activities
25
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kelch-like ECH-associated protein 1 (CHEMBL2069156)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A Comparative Assessment Study of Known Small-Molecule Keap1-Nrf2 Protein-Protein Interaction Inhibitors: Chemical Synthesis, Binding Properties, and Cellular Activity.
Tran KT, Pallesen JS, Solbak SMØ, Narayanan D, Baig A, Zang J, Aguayo-Orozco A, Carmona RMC, Garcia AD, Bach A.
J Med Chem (2019) 62 : 8028 -8052
PubMed 31411465 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 21 7.04 9.00
Inhibition 4 - -
IC50 1 8.10 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3819587 2 9.00
CHEMBL4077198 1 8.68
CHEMBL3237245 1 8.21
CHEMBL4440989 1 7.89
CHEMBL3899754 1 7.32
CHEMBL4534676 1 7.29
CHEMBL3632711 1 7.28
CHEMBL2402207 1 6.60
CHEMBL3423015 1 6.27
CHEMBL2381968 1 6.25
CHEMBL2402206 1 5.55
CHEMBL3235901 1 4.15
CHEMBL3617836 1 -
CHEMBL4167734 1 -
CHEMBL4576082 1 -
CHEMBL4164286 1 -
CHEMBL3586646 1 -
CHEMBL4166691 1 -
CHEMBL3134423 1 -
CHEMBL3235898 1 -
CHEMBL256097 1 -
CHEMBL71595 HYDROGEN PEROXIDE 4.0 1 -
CHEMBL601757 1 -
CHEMBL1239 BENZYL BENZOATE 4.0 1 -
CHEMBL104 CLOTRIMAZOLE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3819587 Ki = 1.0 nM 9.00
CHEMBL4077198 Ki = 2.1 nM 8.68
CHEMBL3237245 Ki = 6.1 nM 8.21
CHEMBL3819587 IC50 = 8.0 nM 8.10
CHEMBL4440989 Ki = 13.0 nM 7.89
CHEMBL3899754 Ki = 48.0 nM 7.32
CHEMBL4534676 Ki = 51.0 nM 7.29
CHEMBL3632711 Ki = 53.0 nM 7.28
CHEMBL2402207 Ki = 250.0 nM 6.60
CHEMBL3423015 Ki = 540.0 nM 6.27
CHEMBL2381968 Ki = 560.0 nM 6.25
CHEMBL2402206 Ki = 2800.0 nM 5.55
CHEMBL3235901 Ki = 71000.0 nM 4.15
CHEMBL104 CLOTRIMAZOLE Inhibition - % -
CHEMBL1239 BENZYL BENZOATE Inhibition - % -
CHEMBL601757 Inhibition - % -
CHEMBL71595 HYDROGEN PEROXIDE Inhibition - % -
CHEMBL256097 Ki <= 200000.0 nM -
CHEMBL3235898 Ki = 280000.0 nM -
CHEMBL3134423 Ki <= 49000.0 nM -
CHEMBL4166691 Ki <= 99000.0 nM -
CHEMBL3586646 Ki <= 800000.0 nM -
CHEMBL4164286 Ki <= 800000.0 nM -
CHEMBL4576082 Ki <= 800000.0 nM -
CHEMBL4167734 Ki <= 77000.0 nM -
CHEMBL3617836 Ki <= 100000.0 nM -